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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermus thermophilus V-type ATP synthase subunit D (atpD) CSB-YP741275TNS
CSB-EP741275TNS
CSB-BP741275TNS
CSB-MP741275TNS
CSB-EP741275TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Valine--tRNA ligase (valS), partial CSB-YP741276TNS
CSB-EP741276TNS
CSB-BP741276TNS
CSB-MP741276TNS
CSB-EP741276TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP741277TNS
CSB-EP741277TNS
CSB-BP741277TNS
CSB-MP741277TNS
CSB-EP741277TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Isoleucine--tRNA ligase (ileS), partial CSB-YP741278TNS
CSB-EP741278TNS
CSB-BP741278TNS
CSB-MP741278TNS
CSB-EP741278TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP741279TNS
CSB-EP741279TNS
CSB-BP741279TNS
CSB-MP741279TNS
CSB-EP741279TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Regulatory protein recX (recX) CSB-YP741280TNS
CSB-EP741280TNS
CSB-BP741280TNS
CSB-MP741280TNS
CSB-EP741280TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP741281TNS
CSB-EP741281TNS
CSB-BP741281TNS
CSB-MP741281TNS
CSB-EP741281TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Probable endonuclease 4 (nfo) CSB-YP741282TNS
CSB-EP741282TNS
CSB-BP741282TNS
CSB-MP741282TNS
CSB-EP741282TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP741283TNS
CSB-EP741283TNS
CSB-BP741283TNS
CSB-MP741283TNS
CSB-EP741283TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus L-threonine 3-dehydrogenase (tdh) CSB-YP741284TNS
CSB-EP741284TNS
CSB-BP741284TNS
CSB-MP741284TNS
CSB-EP741284TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Acyl carrier protein (acpP) CSB-YP741285TNS
CSB-EP741285TNS
CSB-BP741285TNS
CSB-MP741285TNS
CSB-EP741285TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0102 protein TT_C0005 (TT_C0005) CSB-YP741286TNS
CSB-EP741286TNS
CSB-BP741286TNS
CSB-MP741286TNS
CSB-EP741286TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP741287LEV
CSB-EP741287LEV
CSB-BP741287LEV
CSB-MP741287LEV
CSB-EP741287LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Ketol-acid reductoisomerase (ilvC) CSB-YP741288LEV
CSB-EP741288LEV
CSB-BP741288LEV
CSB-MP741288LEV
CSB-EP741288LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Apolipoprotein N-acyltransferase 2 (lnt2), partial CSB-YP741289LEV1
CSB-EP741289LEV1
CSB-BP741289LEV1
CSB-MP741289LEV1
CSB-EP741289LEV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP741290LEV
CSB-EP741290LEV
CSB-BP741290LEV
CSB-MP741290LEV
CSB-EP741290LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Quinolinate synthase A (nadA) CSB-YP741291LEV
CSB-EP741291LEV
CSB-BP741291LEV
CSB-MP741291LEV
CSB-EP741291LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L2 (rplB) CSB-YP741292LEV
CSB-EP741292LEV
CSB-BP741292LEV
CSB-MP741292LEV
CSB-EP741292LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S13 (rpsM) CSB-YP741293LEV
CSB-EP741293LEV
CSB-BP741293LEV
CSB-MP741293LEV
CSB-EP741293LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP741294LEV
CSB-EP741294LEV
CSB-BP741294LEV
CSB-MP741294LEV
CSB-EP741294LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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