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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Candida glabrata Mitochondrial morphogenesis protein SLD7 (SLD7) CSB-YP739435CZI
CSB-EP739435CZI
CSB-BP739435CZI
CSB-MP739435CZI
CSB-EP739435CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 26S proteasome regulatory subunit RPN11 (RPN11) CSB-YP739436CZI
CSB-EP739436CZI
CSB-BP739436CZI
CSB-MP739436CZI
CSB-EP739436CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Phosphoglycerate kinase (PGK1) CSB-YP739437CZI
CSB-EP739437CZI
CSB-BP739437CZI
CSB-MP739437CZI
CSB-EP739437CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Eukaryotic translation initiation factor 3 subunit I (TIF34) CSB-YP739438CZI
CSB-EP739438CZI
CSB-BP739438CZI
CSB-MP739438CZI
CSB-EP739438CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Altered inheritance of mitochondria protein 23, mitochondrial (AIM23) CSB-YP739439CZI
CSB-EP739439CZI
CSB-BP739439CZI
CSB-MP739439CZI
CSB-EP739439CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ribulose-phosphate 3-epimerase (RPE1) CSB-YP739440CZI
CSB-EP739440CZI
CSB-BP739440CZI
CSB-MP739440CZI
CSB-EP739440CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 40S ribosomal protein S23 (RPS23) CSB-YP739441CZI
CSB-EP739441CZI
CSB-BP739441CZI
CSB-MP739441CZI
CSB-EP739441CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mediator of RNA polymerase II transcription subunit 4 (MED4) CSB-YP739442CZI
CSB-EP739442CZI
CSB-BP739442CZI
CSB-MP739442CZI
CSB-EP739442CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ribose-5-phosphate isomerase (RKI1) CSB-YP739443CZI
CSB-EP739443CZI
CSB-BP739443CZI
CSB-MP739443CZI
CSB-EP739443CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata GPI-anchored wall transfer protein 1 (GWT1), partial CSB-YP739444CZI
CSB-EP739444CZI
CSB-BP739444CZI
CSB-MP739444CZI
CSB-EP739444CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Biogenesis of lysosome-related organelles complex 1 subunit BLI1 (BLI1) CSB-YP739445CZI
CSB-EP739445CZI
CSB-BP739445CZI
CSB-MP739445CZI
CSB-EP739445CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata GPI inositol-deacylase (BST1), partial CSB-YP739446CZI
CSB-EP739446CZI
CSB-BP739446CZI
CSB-MP739446CZI
CSB-EP739446CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Autophagy-related protein 31 (CIS1) CSB-YP739447CZI
CSB-EP739447CZI
CSB-BP739447CZI
CSB-MP739447CZI
CSB-EP739447CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine/threonine-protein phosphatase 4 catalytic subunit (PPH3) CSB-YP739448CZI
CSB-EP739448CZI
CSB-BP739448CZI
CSB-MP739448CZI
CSB-EP739448CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata FK506-binding protein 1 (FPR1) CSB-YP739449CZI
CSB-EP739449CZI
CSB-BP739449CZI
CSB-MP739449CZI
CSB-EP739449CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cytochrome c peroxidase, mitochondrial (CAGL0K08184g) CSB-YP739450CZI
CSB-EP739450CZI
CSB-BP739450CZI
CSB-MP739450CZI
CSB-EP739450CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-mRNA-processing ATP-dependent RNA helicase PRP5 (PRP5), partial CSB-YP739451CZI
CSB-EP739451CZI
CSB-BP739451CZI
CSB-MP739451CZI
CSB-EP739451CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata RNA polymerase II transcription factor B subunit 3 (TFB3) CSB-YP739452CZI
CSB-EP739452CZI
CSB-BP739452CZI
CSB-MP739452CZI
CSB-EP739452CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Glutamine synthetase (GLN1) CSB-YP739453CZI
CSB-EP739453CZI
CSB-BP739453CZI
CSB-MP739453CZI
CSB-EP739453CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial import inner membrane translocase subunit TIM21 (TIM21), partial CSB-YP739454CZI1
CSB-EP739454CZI1
CSB-BP739454CZI1
CSB-MP739454CZI1
CSB-EP739454CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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