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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian L-lactate dehydrogenase 1 (ldh1) CSB-YP740195BAAE
CSB-EP740195BAAE
CSB-BP740195BAAE
CSB-MP740195BAAE
CSB-EP740195BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Xanthine phosphoribosyltransferase (xpt) CSB-YP740196BAAE
CSB-EP740196BAAE
CSB-BP740196BAAE
CSB-MP740196BAAE
CSB-EP740196BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0302 protein BT9727_1402 (BT9727_1402) CSB-YP740197BAAE
CSB-EP740197BAAE
CSB-BP740197BAAE
CSB-MP740197BAAE
CSB-EP740197BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Cytidylate kinase (cmk) CSB-YP740198BAAE
CSB-EP740198BAAE
CSB-BP740198BAAE
CSB-MP740198BAAE
CSB-EP740198BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP740199BAAE
CSB-EP740199BAAE
CSB-BP740199BAAE
CSB-MP740199BAAE
CSB-EP740199BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP740200BAAE
CSB-EP740200BAAE
CSB-BP740200BAAE
CSB-MP740200BAAE
CSB-EP740200BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tryptophan synthase beta chain (trpB) CSB-YP740201BAAE
CSB-EP740201BAAE
CSB-BP740201BAAE
CSB-MP740201BAAE
CSB-EP740201BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0413 protein BT9727_1097 (BT9727_1097) CSB-YP740202BAAE
CSB-EP740202BAAE
CSB-BP740202BAAE
CSB-MP740202BAAE
CSB-EP740202BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glycerol kinase (glpK) CSB-YP740203BAAE
CSB-EP740203BAAE
CSB-BP740203BAAE
CSB-MP740203BAAE
CSB-EP740203BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Protein translocase subunit SecA 2 (secA2), partial CSB-YP740204BAAE
CSB-EP740204BAAE
CSB-BP740204BAAE
CSB-MP740204BAAE
CSB-EP740204BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Small, acid-soluble spore protein H (sspH) CSB-YP740205BAAE
CSB-EP740205BAAE
CSB-BP740205BAAE
CSB-MP740205BAAE
CSB-EP740205BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0295 protein BT9727_0449 (BT9727_0449), partial CSB-YP740206BAAE1
CSB-EP740206BAAE1
CSB-BP740206BAAE1
CSB-MP740206BAAE1
CSB-EP740206BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ornithine carbamoyltransferase, catabolic (arcB) CSB-YP740207BAAE
CSB-EP740207BAAE
CSB-BP740207BAAE
CSB-MP740207BAAE
CSB-EP740207BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA ligase (ligA), partial CSB-YP740208BAAE
CSB-EP740208BAAE
CSB-BP740208BAAE
CSB-MP740208BAAE
CSB-EP740208BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Redox-sensing transcriptional repressor rex (rex) CSB-YP740209BAAE
CSB-EP740209BAAE
CSB-BP740209BAAE
CSB-MP740209BAAE
CSB-EP740209BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian HTH-type transcriptional regulator CzcR (czcR) CSB-YP740210BAAE
CSB-EP740210BAAE
CSB-BP740210BAAE
CSB-MP740210BAAE
CSB-EP740210BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP740211BAAE
CSB-EP740211BAAE
CSB-BP740211BAAE
CSB-MP740211BAAE
CSB-EP740211BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Adenylate kinase (adk) CSB-YP740212BAAE
CSB-EP740212BAAE
CSB-BP740212BAAE
CSB-MP740212BAAE
CSB-EP740212BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L6 (rplF) CSB-YP740213BAAE
CSB-EP740213BAAE
CSB-BP740213BAAE
CSB-MP740213BAAE
CSB-EP740213BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L22 (rplV) CSB-YP740214BAAE
CSB-EP740214BAAE
CSB-BP740214BAAE
CSB-MP740214BAAE
CSB-EP740214BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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