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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP740395PIG
CSB-EP740395PIG
CSB-BP740395PIG
CSB-MP740395PIG
CSB-EP740395PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP740396PIG
CSB-EP740396PIG
CSB-BP740396PIG
CSB-MP740396PIG
CSB-EP740396PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Chaperone protein TorD (torD) CSB-YP740397PIG
CSB-EP740397PIG
CSB-BP740397PIG
CSB-MP740397PIG
CSB-EP740397PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP740398PIG
CSB-EP740398PIG
CSB-BP740398PIG
CSB-MP740398PIG
CSB-EP740398PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Elongation factor G 2 (fusA2), partial CSB-YP740399PIG
CSB-EP740399PIG
CSB-BP740399PIG
CSB-MP740399PIG
CSB-EP740399PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L32 (rpmF) CSB-YP740400PIG
CSB-EP740400PIG
CSB-BP740400PIG
CSB-MP740400PIG
CSB-EP740400PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP740401PIG
CSB-EP740401PIG
CSB-BP740401PIG
CSB-MP740401PIG
CSB-EP740401PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Cysteine--tRNA ligase 1 (cysS1) CSB-YP740402PIG
CSB-EP740402PIG
CSB-BP740402PIG
CSB-MP740402PIG
CSB-EP740402PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Probable septum site-determining protein MinC (minC) CSB-YP740403PIG
CSB-EP740403PIG
CSB-BP740403PIG
CSB-MP740403PIG
CSB-EP740403PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ferrochelatase (hemH) CSB-YP740404PIG
CSB-EP740404PIG
CSB-BP740404PIG
CSB-MP740404PIG
CSB-EP740404PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP740405PIG
CSB-EP740405PIG
CSB-BP740405PIG
CSB-MP740405PIG
CSB-EP740405PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Chemotaxis response regulator protein-glutamate methylesterase 2 (cheB2) CSB-YP740406PIG
CSB-EP740406PIG
CSB-BP740406PIG
CSB-MP740406PIG
CSB-EP740406PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Gamma-glutamyl phosphate reductase (proA) CSB-YP740407PIG
CSB-EP740407PIG
CSB-BP740407PIG
CSB-MP740407PIG
CSB-EP740407PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP740408PIG
CSB-EP740408PIG
CSB-BP740408PIG
CSB-MP740408PIG
CSB-EP740408PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Serine hydroxymethyltransferase 1 (glyA1) CSB-YP740409PIG
CSB-EP740409PIG
CSB-BP740409PIG
CSB-MP740409PIG
CSB-EP740409PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP740410PIG
CSB-EP740410PIG
CSB-BP740410PIG
CSB-MP740410PIG
CSB-EP740410PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Chaperone protein hscA homolog (hscA), partial CSB-YP740411PIG
CSB-EP740411PIG
CSB-BP740411PIG
CSB-MP740411PIG
CSB-EP740411PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum SsrA-binding protein (smpB) CSB-YP740412PIG
CSB-EP740412PIG
CSB-BP740412PIG
CSB-MP740412PIG
CSB-EP740412PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phosphopentomutase (deoB) CSB-YP740413PIG
CSB-EP740413PIG
CSB-BP740413PIG
CSB-MP740413PIG
CSB-EP740413PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA pseudouridine synthase B (truB) CSB-YP740414PIG
CSB-EP740414PIG
CSB-BP740414PIG
CSB-MP740414PIG
CSB-EP740414PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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