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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio Zinc transporter 6 (slc30a6), partial CSB-YP740695DIL1
CSB-EP740695DIL1
CSB-BP740695DIL1
CSB-MP740695DIL1
CSB-EP740695DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transcription factor AP-2-epsilon (tfap2e) CSB-YP740696DIL
CSB-EP740696DIL
CSB-BP740696DIL
CSB-MP740696DIL
CSB-EP740696DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Microtubule-associated serine/threonine-protein kinase 2 (MAST2), partial CSB-YP740697HU
CSB-EP740697HU
CSB-BP740697HU
CSB-MP740697HU
CSB-EP740697HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Tax1-binding protein 1 homolog B (tax1bp1b), partial CSB-YP740698DIL
CSB-EP740698DIL
CSB-BP740698DIL
CSB-MP740698DIL
CSB-EP740698DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Seizure 6-like protein (Sez6l), partial CSB-YP740699MO
CSB-EP740699MO
CSB-BP740699MO
CSB-MP740699MO
CSB-EP740699MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse UPF0536 protein C12orf66 homolog CSB-YP740700MO
CSB-EP740700MO
CSB-BP740700MO
CSB-MP740700MO
CSB-EP740700MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Heme transporter HRG1 (SLC48A1), partial CSB-YP740701HU1
CSB-EP740701HU1
CSB-BP740701HU1
CSB-MP740701HU1
CSB-EP740701HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Large ribosomal subunit protein uL14m (MRPL14) CSB-YP740702HU
CSB-EP740702HU
CSB-BP740702HU
CSB-MP740702HU
CSB-EP740702HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Methyltransferase-like protein 2B (METTL2B) CSB-YP740703HU
CSB-EP740703HU
CSB-BP740703HU
CSB-MP740703HU
CSB-EP740703HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein downstream neighbor of son homolog (donson), partial CSB-YP740704XBF
CSB-EP740704XBF
CSB-BP740704XBF
CSB-MP740704XBF
CSB-EP740704XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C1orf94 (C1orf94) CSB-YP740705HU
CSB-EP740705HU
CSB-BP740705HU
CSB-MP740705HU
CSB-EP740705HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 1 homolog (ZFP1) CSB-YP740706HU
CSB-EP740706HU
CSB-BP740706HU
CSB-MP740706HU
CSB-EP740706HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein ADORA3, isoform 3 (ADORA3), partial CSB-YP740707HU1
CSB-EP740707HU1
CSB-BP740707HU1
CSB-MP740707HU1
CSB-EP740707HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Arginine and glutamate-rich protein 1 (arglu1) CSB-YP740708XBF
CSB-EP740708XBF
CSB-BP740708XBF
CSB-MP740708XBF
CSB-EP740708XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Basic leucine zipper and W2 domain-containing protein 1 (bzw1) CSB-YP740709XBF
CSB-EP740709XBF
CSB-BP740709XBF
CSB-MP740709XBF
CSB-EP740709XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein YIF1B (yif1b), partial CSB-YP740710XBF1
CSB-EP740710XBF1
CSB-BP740710XBF1
CSB-MP740710XBF1
CSB-EP740710XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse V-set and immunoglobulin domain-containing protein 8 (Vsig8), partial CSB-YP740711MO
CSB-EP740711MO
CSB-BP740711MO
CSB-MP740711MO
CSB-EP740711MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis BTB/POZ domain-containing protein kctd15 (kctd15) CSB-YP740712XBF
CSB-EP740712XBF
CSB-BP740712XBF
CSB-MP740712XBF
CSB-EP740712XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase Nek5 (NEK5) CSB-YP740713HU
CSB-EP740713HU
CSB-BP740713HU
CSB-MP740713HU
CSB-EP740713HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Podocan-like protein 1 (Podnl1) CSB-YP740714MO
CSB-EP740714MO
CSB-BP740714MO
CSB-MP740714MO
CSB-EP740714MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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