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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Protein FAM54A (FAM54A) CSB-YP740715HU
CSB-EP740715HU
CSB-BP740715HU
CSB-MP740715HU
CSB-EP740715HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ferredoxin-2, mitochondrial (FDX2) CSB-YP740716HU
CSB-EP740716HU
CSB-BP740716HU
CSB-MP740716HU
CSB-EP740716HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis WD40 repeat-containing protein SMU1 (smu1), partial CSB-YP740717XBF
CSB-EP740717XBF
CSB-BP740717XBF
CSB-MP740717XBF
CSB-EP740717XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Nuclear factor related to kappa-B-binding protein (nfrkb), partial CSB-YP740718XBF
CSB-EP740718XBF
CSB-BP740718XBF
CSB-MP740718XBF
CSB-EP740718XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (inpp5d), partial CSB-YP740719XBE
CSB-EP740719XBE
CSB-BP740719XBE
CSB-MP740719XBE
CSB-EP740719XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase znrf1 (znrf1) CSB-YP740720DIL
CSB-EP740720DIL
CSB-BP740720DIL
CSB-MP740720DIL
CSB-EP740720DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Carnitine O-palmitoyltransferase 2, mitochondrial (cpt2), partial CSB-YP740721XBF
CSB-EP740721XBF
CSB-BP740721XBF
CSB-MP740721XBF
CSB-EP740721XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C17orf63 homolog CSB-YP740722MO
CSB-EP740722MO
CSB-BP740722MO
CSB-MP740722MO
CSB-EP740722MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Single-strand selective monofunctional uracil DNA glycosylase (Smug1) CSB-YP740723MO
CSB-EP740723MO
CSB-BP740723MO
CSB-MP740723MO
CSB-EP740723MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 60S ribosomal protein L22-like 1 (RPL22L1) CSB-YP740724HU
CSB-EP740724HU
CSB-BP740724HU
CSB-MP740724HU
CSB-EP740724HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Pre-mRNA-splicing factor RBM22 (rbm22) CSB-YP740725XBF
CSB-EP740725XBF
CSB-BP740725XBF
CSB-MP740725XBF
CSB-EP740725XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein wntless homolog (Wls), partial CSB-YP740726RA1
CSB-EP740726RA1
CSB-BP740726RA1
CSB-MP740726RA1
CSB-EP740726RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Bombesin receptor-activated protein C6orf89 homolog, partial CSB-YP740727RA1
CSB-EP740727RA1
CSB-BP740727RA1
CSB-MP740727RA1
CSB-EP740727RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse O-phosphoseryl-tRNA (Sec) selenium transferase (Sepsecs) CSB-YP740728MO
CSB-EP740728MO
CSB-BP740728MO
CSB-MP740728MO
CSB-EP740728MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Dihydrolipoyl dehydrogenase, mitochondrial (Dld) CSB-YP740729RA
CSB-EP740729RA
CSB-BP740729RA
CSB-MP740729RA
CSB-EP740729RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tail-anchored protein insertion receptor WRB (Wrb), partial CSB-YP740730RA1
CSB-EP740730RA1
CSB-BP740730RA1
CSB-MP740730RA1
CSB-EP740730RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Echinoderm microtubule-associated protein-like 2 (Eml2) CSB-YP740731RA
CSB-EP740731RA
CSB-BP740731RA
CSB-MP740731RA
CSB-EP740731RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA damage-binding protein 1 (ddb1), partial CSB-YP740732XBE
CSB-EP740732XBE
CSB-BP740732XBE
CSB-MP740732XBE
CSB-EP740732XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Proteolipid protein 2 (Plp2), partial CSB-YP740733RA1
CSB-EP740733RA1
CSB-BP740733RA1
CSB-MP740733RA1
CSB-EP740733RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Pituitary tumor-transforming gene 1 protein-interacting protein (Pttg1ip), partial CSB-YP740734RA
CSB-EP740734RA
CSB-BP740734RA
CSB-MP740734RA
CSB-EP740734RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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