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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant NAD (P)H-quinone oxidoreductase subunit I, chloroplastic (ndhI) CSB-YP741115AIQ
CSB-EP741115AIQ
CSB-BP741115AIQ
CSB-MP741115AIQ
CSB-EP741115AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S3, chloroplastic (rps3) CSB-YP741116AIQ
CSB-EP741116AIQ
CSB-BP741116AIQ
CSB-MP741116AIQ
CSB-EP741116AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b6-f complex subunit 4 (petD), partial CSB-YP741117AIQ1
CSB-EP741117AIQ1
CSB-BP741117AIQ1
CSB-MP741117AIQ1
CSB-EP741117AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP741118AIQ
CSB-EP741118AIQ
CSB-BP741118AIQ
CSB-MP741118AIQ
CSB-EP741118AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP741119AIQ
CSB-EP741119AIQ
CSB-BP741119AIQ
CSB-MP741119AIQ
CSB-EP741119AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP741120AIQ
CSB-EP741120AIQ
CSB-BP741120AIQ
CSB-MP741120AIQ
CSB-EP741120AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyclopes didactylus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP741121CHAF
CSB-EP741121CHAF
CSB-BP741121CHAF
CSB-MP741121CHAF
CSB-EP741121CHAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein (PREX2), partial CSB-YP741122HU
CSB-EP741122HU
CSB-BP741122HU
CSB-MP741122HU
CSB-EP741122HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vulpes vulpes Cytochrome c oxidase subunit 5B, mitochondrial (COX5B) CSB-YP741123VFW
CSB-EP741123VFW
CSB-BP741123VFW
CSB-MP741123VFW
CSB-EP741123VFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Jaculus jaculus Cytochrome b (MT-CYB), partial CSB-YP741124JAAD
CSB-EP741124JAAD
CSB-BP741124JAAD
CSB-MP741124JAAD
CSB-EP741124JAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus pinaster Histone H3.3 CSB-YP741125PKM
CSB-EP741125PKM
CSB-BP741125PKM
CSB-MP741125PKM
CSB-EP741125PKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsular polysaccharide phosphotransferase fcs1 (fcs1) CSB-YP741126HSZ
CSB-EP741126HSZ
CSB-BP741126HSZ
CSB-MP741126HSZ
CSB-EP741126HSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TD and POZ domain-containing protein 3 (Tdpoz3) CSB-YP741127MO
CSB-EP741127MO
CSB-BP741127MO
CSB-MP741127MO
CSB-EP741127MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Activator of 90 kDa heat shock protein ATPase homolog 2 (AHSA2) CSB-YP741128HU
CSB-EP741128HU
CSB-BP741128HU
CSB-MP741128HU
CSB-EP741128HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Neuropeptide-like 3 (Nplp3) CSB-YP741129DMR
CSB-EP741129DMR
CSB-BP741129DMR
CSB-MP741129DMR
CSB-EP741129DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Viverra megaspila Cytochrome b (MT-CYB), partial CSB-YP741130VAAR
CSB-EP741130VAAR
CSB-BP741130VAAR
CSB-MP741130VAAR
CSB-EP741130VAAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epinephelus coioides 60S ribosomal protein L41 (rpl41) CSB-YP741131EEU
CSB-EP741131EEU
CSB-BP741131EEU
CSB-MP741131EEU
CSB-EP741131EEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizopus oryzae Orotidine 5'-phosphate decarboxylase (pyrG) CSB-YP741132RDE
CSB-EP741132RDE
CSB-BP741132RDE
CSB-MP741132RDE
CSB-EP741132RDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorokybus atmophyticus Protein psbN (psbN), partial CSB-YP741133CWP
CSB-EP741133CWP
CSB-BP741133CWP
CSB-MP741133CWP
CSB-EP741133CWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Fibrinogen-like protein 1 (Fgl1) CSB-YP741134MO
CSB-BP741134MO
CSB-MP741134MO
CSB-EP741134MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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