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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S14 type Z (rpsZ) CSB-YP741155LAAD
CSB-EP741155LAAD
CSB-BP741155LAAD
CSB-MP741155LAAD
CSB-EP741155LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L30 (rpmD) CSB-YP741156LAAD
CSB-EP741156LAAD
CSB-BP741156LAAD
CSB-MP741156LAAD
CSB-EP741156LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S11 (rpsK) CSB-YP741157LAAD
CSB-EP741157LAAD
CSB-BP741157LAAD
CSB-MP741157LAAD
CSB-EP741157LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protein FdhD homolog (fdhD) CSB-YP741158LAAD
CSB-EP741158LAAD
CSB-BP741158LAAD
CSB-MP741158LAAD
CSB-EP741158LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Thymidine kinase (tdk) CSB-YP741159LAAD
CSB-EP741159LAAD
CSB-BP741159LAAD
CSB-MP741159LAAD
CSB-EP741159LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b ATP synthase subunit a (atpB), partial CSB-YP741160LAAD1
CSB-EP741160LAAD1
CSB-BP741160LAAD1
CSB-MP741160LAAD1
CSB-EP741160LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b ATP synthase epsilon chain (atpC) CSB-YP741161LAAD
CSB-EP741161LAAD
CSB-BP741161LAAD
CSB-MP741161LAAD
CSB-EP741161LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP741162LAAD
CSB-EP741162LAAD
CSB-BP741162LAAD
CSB-MP741162LAAD
CSB-EP741162LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b NADPH dehydrogenase (namA) CSB-YP741163LAAD
CSB-EP741163LAAD
CSB-BP741163LAAD
CSB-MP741163LAAD
CSB-EP741163LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0349 protein LMOf2365_2366 (LMOf2365_2366) CSB-YP741164LAAD
CSB-EP741164LAAD
CSB-BP741164LAAD
CSB-MP741164LAAD
CSB-EP741164LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP741165LAAD
CSB-EP741165LAAD
CSB-BP741165LAAD
CSB-MP741165LAAD
CSB-EP741165LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0342 protein LMOf2365_2256 (LMOf2365_2256) CSB-YP741166LAAD
CSB-EP741166LAAD
CSB-BP741166LAAD
CSB-MP741166LAAD
CSB-EP741166LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0324 membrane protein LMOf2365_2179 (LMOf2365_2179), partial CSB-YP741167LAAD
CSB-EP741167LAAD
CSB-BP741167LAAD
CSB-MP741167LAAD
CSB-EP741167LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP741168LAAD
CSB-EP741168LAAD
CSB-BP741168LAAD
CSB-MP741168LAAD
CSB-EP741168LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 60 kDa chaperonin (groL), partial CSB-YP741169LAAD
CSB-EP741169LAAD
CSB-BP741169LAAD
CSB-MP741169LAAD
CSB-EP741169LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphopantetheine adenylyltransferase (coaD) CSB-YP741170LAAD
CSB-EP741170LAAD
CSB-BP741170LAAD
CSB-MP741170LAAD
CSB-EP741170LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP741171LAAD
CSB-EP741171LAAD
CSB-BP741171LAAD
CSB-MP741171LAAD
CSB-EP741171LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Cell division protein SepF (sepF) CSB-YP741172LAAD
CSB-EP741172LAAD
CSB-BP741172LAAD
CSB-MP741172LAAD
CSB-EP741172LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP741173LAAD
CSB-EP741173LAAD
CSB-BP741173LAAD
CSB-MP741173LAAD
CSB-EP741173LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Dihydroxy-acid dehydratase (ilvD), partial CSB-YP741174LAAD
CSB-EP741174LAAD
CSB-BP741174LAAD
CSB-MP741174LAAD
CSB-EP741174LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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