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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Aldehyde dehydrogenase family 3 member H1 (ALDH3H1) CSB-YP741095DOA
CSB-EP741095DOA
CSB-BP741095DOA
CSB-MP741095DOA
CSB-EP741095DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sushi, nidogen and EGF-like domain-containing protein 1 (Sned1), partial CSB-YP741096MO
CSB-EP741096MO
CSB-BP741096MO
CSB-MP741096MO
CSB-EP741096MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 45 (USP45), partial CSB-YP741097HU
CSB-EP741097HU
CSB-BP741097HU
CSB-MP741097HU
CSB-EP741097HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human MOB kinase activator 3C (MOB3C) CSB-YP741098HU
CSB-EP741098HU
CSB-BP741098HU
CSB-MP741098HU
CSB-EP741098HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF110 (ERF110) CSB-YP741099DOA
CSB-EP741099DOA
CSB-BP741099DOA
CSB-MP741099DOA
CSB-EP741099DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconobacter oxydans Glycerol dehydrogenase small subunit (sldB), partial CSB-YP741100GGU1
CSB-EP741100GGU1
CSB-BP741100GGU1
CSB-MP741100GGU1
CSB-EP741100GGU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus amyloliquefaciens 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP741101BQB
CSB-EP741101BQB
CSB-BP741101BQB
CSB-MP741101BQB
CSB-EP741101BQB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Protein RAFTIN 1C (RAFTIN1C) CSB-YP741102TQN
CSB-EP741102TQN
CSB-BP741102TQN
CSB-MP741102TQN
CSB-EP741102TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccase-1 (LAC1), partial CSB-YP741103MFAF
CSB-EP741103MFAF
CSB-BP741103MFAF
CSB-MP741103MFAF
CSB-EP741103MFAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF274 (ORF274) CSB-YP741104AFAE
CSB-EP741104AFAE
CSB-BP741104AFAE
CSB-MP741104AFAE
CSB-EP741104AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF140 (ORF140) CSB-YP741105AFAE
CSB-EP741105AFAE
CSB-BP741105AFAE
CSB-MP741105AFAE
CSB-EP741105AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF195 (ORF195) CSB-YP741106AFAE
CSB-EP741106AFAE
CSB-BP741106AFAE
CSB-MP741106AFAE
CSB-EP741106AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF74 (ORF74) CSB-YP741107AFAE
CSB-EP741107AFAE
CSB-BP741107AFAE
CSB-MP741107AFAE
CSB-EP741107AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Linear gramicidin synthase subunit D (lgrD), partial CSB-YP741108BWM
CSB-EP741108BWM
CSB-BP741108BWM
CSB-MP741108BWM
CSB-EP741108BWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine/threonine-protein kinase Smg1 (Smg1), partial CSB-YP741109DLU
CSB-EP741109DLU
CSB-BP741109DLU
CSB-MP741109DLU
CSB-EP741109DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hyperoodon ampullatus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP741110HDAF1
CSB-EP741110HDAF1
CSB-BP741110HDAF1
CSB-MP741110HDAF1
CSB-EP741110HDAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mannose-1-phosphate guanyltransferase (MPG1) CSB-YP741111KBK
CSB-EP741111KBK
CSB-BP741111KBK
CSB-MP741111KBK
CSB-EP741111KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chaperone protein DnaJ (dnaJ) CSB-YP741112FDR
CSB-EP741112FDR
CSB-BP741112FDR
CSB-MP741112FDR
CSB-EP741112FDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S12, chloroplastic (rps12) CSB-YP741113AIQ
CSB-EP741113AIQ
CSB-BP741113AIQ
CSB-MP741113AIQ
CSB-EP741113AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23, chloroplastic (rpl23-A) CSB-YP741114AIQ
CSB-EP741114AIQ
CSB-BP741114AIQ
CSB-MP741114AIQ
CSB-EP741114AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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