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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Gamma-glutamyl phosphate reductase (proA) CSB-YP741295LEV
CSB-EP741295LEV
CSB-BP741295LEV
CSB-MP741295LEV
CSB-EP741295LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Methylglyoxal synthase (mgsA) CSB-YP741296LEV
CSB-EP741296LEV
CSB-BP741296LEV
CSB-MP741296LEV
CSB-EP741296LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Phosphomethylpyrimidine synthase (thiC) CSB-YP741297LEV
CSB-EP741297LEV
CSB-BP741297LEV
CSB-MP741297LEV
CSB-EP741297LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Anthranilate phosphoribosyltransferase (trpD) CSB-YP741298LEV
CSB-EP741298LEV
CSB-BP741298LEV
CSB-MP741298LEV
CSB-EP741298LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 50S ribosomal protein L35 (rpmI) CSB-YP741299LEV
CSB-EP741299LEV
CSB-BP741299LEV
CSB-MP741299LEV
CSB-EP741299LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GTPase Der (der) CSB-YP741300LEV
CSB-EP741300LEV
CSB-BP741300LEV
CSB-MP741300LEV
CSB-EP741300LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Glycine--tRNA ligase (glyQS) CSB-YP741301LEV
CSB-EP741301LEV
CSB-BP741301LEV
CSB-MP741301LEV
CSB-EP741301LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable rRNA maturation factor (LIC_12105) CSB-YP741302LEV
CSB-EP741302LEV
CSB-BP741302LEV
CSB-MP741302LEV
CSB-EP741302LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Asparagine--tRNA ligase (asnS) CSB-YP741303LEV
CSB-EP741303LEV
CSB-BP741303LEV
CSB-MP741303LEV
CSB-EP741303LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP741304LEV
CSB-EP741304LEV
CSB-BP741304LEV
CSB-MP741304LEV
CSB-EP741304LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Probable GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP741305LEV
CSB-EP741305LEV
CSB-BP741305LEV
CSB-MP741305LEV
CSB-EP741305LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP741306LEV
CSB-EP741306LEV
CSB-BP741306LEV
CSB-MP741306LEV
CSB-EP741306LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Acetylornithine aminotransferase (argD) CSB-YP741307LEV
CSB-EP741307LEV
CSB-BP741307LEV
CSB-MP741307LEV
CSB-EP741307LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni 30S ribosomal protein S21 (rpsU) CSB-YP741308LEV
CSB-EP741308LEV
CSB-BP741308LEV
CSB-MP741308LEV
CSB-EP741308LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP741309LEV
CSB-EP741309LEV
CSB-BP741309LEV
CSB-MP741309LEV
CSB-EP741309LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP741310LEV
CSB-EP741310LEV
CSB-BP741310LEV
CSB-MP741310LEV
CSB-EP741310LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni HPr kinase/phosphorylase (hprK) CSB-YP741311LEV
CSB-EP741311LEV
CSB-BP741311LEV
CSB-MP741311LEV
CSB-EP741311LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP741312LEV
CSB-EP741312LEV
CSB-BP741312LEV
CSB-MP741312LEV
CSB-EP741312LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni S-adenosylmethionine synthase (metK) CSB-YP741313LEV
CSB-EP741313LEV
CSB-BP741313LEV
CSB-MP741313LEV
CSB-EP741313LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni UPF0176 protein LIC_10976 (LIC_10976) CSB-YP741314LEV
CSB-EP741314LEV
CSB-BP741314LEV
CSB-MP741314LEV
CSB-EP741314LEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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