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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 3 subunit M (Tango7) CSB-YP741956DLU
CSB-EP741956DLU
CSB-BP741956DLU
CSB-MP741956DLU
CSB-EP741956DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Vacuolar protein-sorting-associated protein 25 (Vps25) CSB-YP741957DLU
CSB-EP741957DLU
CSB-BP741957DLU
CSB-MP741957DLU
CSB-EP741957DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein CLP1 homolog (cbc) CSB-YP741958DLU
CSB-EP741958DLU
CSB-BP741958DLU
CSB-MP741958DLU
CSB-EP741958DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein crossbronx (cbx) CSB-YP741959DLU
CSB-EP741959DLU
CSB-BP741959DLU
CSB-MP741959DLU
CSB-EP741959DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Flap endonuclease 1 (Fen1) CSB-YP741960DLU
CSB-EP741960DLU
CSB-BP741960DLU
CSB-MP741960DLU
CSB-EP741960DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Phosphoglucomutase (Pgm), partial CSB-YP741961DMJ
CSB-EP741961DMJ
CSB-BP741961DMJ
CSB-MP741961DMJ
CSB-EP741961DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Cysteine--tRNA ligase, cytoplasmic (Aats-cys), partial CSB-YP741962DLU
CSB-EP741962DLU
CSB-BP741962DLU
CSB-MP741962DLU
CSB-EP741962DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Probable cytochrome P450 12d1 distal, mitochondrial (Cyp12d1-d), partial CSB-YP741963DLU
CSB-EP741963DLU
CSB-BP741963DLU
CSB-MP741963DLU
CSB-EP741963DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 40S ribosomal protein S19b (RpS19b) CSB-YP741964DLU
CSB-EP741964DLU
CSB-BP741964DLU
CSB-MP741964DLU
CSB-EP741964DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Stellate protein CG33238 (Ste:CG33238) CSB-YP741965DLU
CSB-EP741965DLU
CSB-BP741965DLU
CSB-MP741965DLU
CSB-EP741965DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Probable H/ACA ribonucleoprotein complex subunit 1 (CG4038) CSB-YP741966DLU
CSB-EP741966DLU
CSB-BP741966DLU
CSB-MP741966DLU
CSB-EP741966DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Inosine-uridine-preferring nucleoside hydrolase homolog (iunH) CSB-YP741967DKK
CSB-EP741967DKK
CSB-BP741967DKK
CSB-MP741967DKK
CSB-EP741967DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Expansin-like protein 3 (expl3), partial CSB-YP741968DKK
CSB-EP741968DKK
CSB-BP741968DKK
CSB-MP741968DKK
CSB-EP741968DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Putative exosome complex component rrp40 (exosc3) CSB-YP741969DKK
CSB-EP741969DKK
CSB-BP741969DKK
CSB-MP741969DKK
CSB-EP741969DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human BTB/POZ domain-containing protein KCTD9 (KCTD9) CSB-YP741970HU
CSB-EP741970HU
CSB-BP741970HU
CSB-MP741970HU
CSB-EP741970HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human BEN domain-containing protein 5 (BEND5) CSB-YP741971HU
CSB-EP741971HU
CSB-BP741971HU
CSB-MP741971HU
CSB-EP741971HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human G2/M phase-specific E3 ubiquitin-protein ligase (G2E3) CSB-YP741972HU
CSB-EP741972HU
CSB-BP741972HU
CSB-MP741972HU
CSB-EP741972HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Synaptotagmin-13 (SYT13), partial CSB-YP741973HU
CSB-EP741973HU
CSB-BP741973HU
CSB-MP741973HU
CSB-EP741973HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Solute carrier family 26 member 9 (SLC26A9), partial CSB-YP741974HU
CSB-EP741974HU
CSB-BP741974HU
CSB-MP741974HU
CSB-EP741974HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human CXXC-type zinc finger protein 5 (CXXC5) CSB-YP741975HU
CSB-EP741975HU
CSB-BP741975HU
CSB-MP741975HU
CSB-EP741975HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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