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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Porphyromonas gingivalis 30S ribosomal protein S18 (rpsR) CSB-YP742156EYA
CSB-EP742156EYA
CSB-BP742156EYA
CSB-MP742156EYA
CSB-EP742156EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP742157EYA
CSB-EP742157EYA
CSB-BP742157EYA
CSB-MP742157EYA
CSB-EP742157EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis SsrA-binding protein (smpB) CSB-YP742158EYA
CSB-EP742158EYA
CSB-BP742158EYA
CSB-MP742158EYA
CSB-EP742158EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP742159EYA
CSB-EP742159EYA
CSB-BP742159EYA
CSB-MP742159EYA
CSB-EP742159EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis 50S ribosomal protein L1 (rplA) CSB-YP742160EYA
CSB-EP742160EYA
CSB-BP742160EYA
CSB-MP742160EYA
CSB-EP742160EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Aspartate carbamoyltransferase (pyrB) CSB-YP742161EYA
CSB-EP742161EYA
CSB-BP742161EYA
CSB-MP742161EYA
CSB-EP742161EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Translation initiation factor IF-2 (infB), partial CSB-YP742162EYA
CSB-EP742162EYA
CSB-BP742162EYA
CSB-MP742162EYA
CSB-EP742162EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP742163EYA
CSB-EP742163EYA
CSB-BP742163EYA
CSB-MP742163EYA
CSB-EP742163EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Ferrochelatase (hemH) CSB-YP742164EYA
CSB-EP742164EYA
CSB-BP742164EYA
CSB-MP742164EYA
CSB-EP742164EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP742165EYA
CSB-EP742165EYA
CSB-BP742165EYA
CSB-MP742165EYA
CSB-EP742165EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Redox-sensing transcriptional repressor rex (rex) CSB-YP742166EYA
CSB-EP742166EYA
CSB-BP742166EYA
CSB-MP742166EYA
CSB-EP742166EYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Nucleoid occlusion factor SlmA (slmA) CSB-YP742167PIJ
CSB-EP742167PIJ
CSB-BP742167PIJ
CSB-MP742167PIJ
CSB-EP742167PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP742168PIJ
CSB-EP742168PIJ
CSB-BP742168PIJ
CSB-MP742168PIJ
CSB-EP742168PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP742169PIJ
CSB-EP742169PIJ
CSB-BP742169PIJ
CSB-MP742169PIJ
CSB-EP742169PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Acetylglutamate kinase (argB) CSB-YP742170PIJ
CSB-EP742170PIJ
CSB-BP742170PIJ
CSB-MP742170PIJ
CSB-EP742170PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L3 (rplC) CSB-YP742171PIJ
CSB-EP742171PIJ
CSB-BP742171PIJ
CSB-MP742171PIJ
CSB-EP742171PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S17 (rpsQ) CSB-YP742172PIJ
CSB-EP742172PIJ
CSB-BP742172PIJ
CSB-MP742172PIJ
CSB-EP742172PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S5 (rpsE) CSB-YP742173PIJ
CSB-EP742173PIJ
CSB-BP742173PIJ
CSB-MP742173PIJ
CSB-EP742173PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Peptide deformylase (def) CSB-YP742174PIJ
CSB-EP742174PIJ
CSB-BP742174PIJ
CSB-MP742174PIJ
CSB-EP742174PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP-dependent RNA helicase rhlB (rhlB) CSB-YP742175PIJ
CSB-EP742175PIJ
CSB-BP742175PIJ
CSB-MP742175PIJ
CSB-EP742175PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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