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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cell division protein SepF (sepF) CSB-YP742557MVH
CSB-EP742557MVH
CSB-BP742557MVH
CSB-MP742557MVH
CSB-EP742557MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP742558MVH
CSB-EP742558MVH
CSB-BP742558MVH
CSB-MP742558MVH
CSB-EP742558MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease PH (rph) CSB-YP742559MVH
CSB-EP742559MVH
CSB-BP742559MVH
CSB-MP742559MVH
CSB-EP742559MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant FO synthase (fbiC), partial CSB-YP742560MVH
CSB-EP742560MVH
CSB-BP742560MVH
CSB-MP742560MVH
CSB-EP742560MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Signal transduction histidine-protein kinase/phosphatase mprB (mprB), partial CSB-YP742561MVH
CSB-EP742561MVH
CSB-BP742561MVH
CSB-MP742561MVH
CSB-EP742561MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycothiol acetyltransferase (mshD) CSB-YP742562MVH
CSB-EP742562MVH
CSB-BP742562MVH
CSB-MP742562MVH
CSB-EP742562MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyclopropane mycolic acid synthase MmaA2 (cmaC) CSB-YP742563MVH
CSB-EP742563MVH
CSB-BP742563MVH
CSB-MP742563MVH
CSB-EP742563MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP742564MVH
CSB-EP742564MVH
CSB-BP742564MVH
CSB-MP742564MVH
CSB-EP742564MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant R2-like ligand binding oxidase (Mb0238) CSB-YP742565MVH
CSB-EP742565MVH
CSB-BP742565MVH
CSB-MP742565MVH
CSB-EP742565MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0232 protein Mb0004 (Mb0004) CSB-YP742566MVH
CSB-EP742566MVH
CSB-BP742566MVH
CSB-MP742566MVH
CSB-EP742566MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter hepaticus tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP742567HCO
CSB-EP742567HCO
CSB-BP742567HCO
CSB-MP742567HCO
CSB-EP742567HCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus ducreyi tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP742568HSY
CSB-EP742568HSY
CSB-BP742568HSY
CSB-MP742568HSY
CSB-EP742568HSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 10 kDa chaperonin (groS) CSB-YP742569BHM
CSB-EP742569BHM
CSB-BP742569BHM
CSB-MP742569BHM
CSB-EP742569BHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis tRNA pseudouridine synthase A (truA) CSB-YP742570BUA
CSB-EP742570BUA
CSB-BP742570BUA
CSB-MP742570BUA
CSB-EP742570BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP742571BIK
CSB-EP742571BIK
CSB-BP742571BIK
CSB-MP742571BIK
CSB-EP742571BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP742572SSL
CSB-EP742572SSL
CSB-BP742572SSL
CSB-MP742572SSL
CSB-EP742572SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Urease subunit alpha (ureC), partial CSB-YP742573SSL
CSB-EP742573SSL
CSB-BP742573SSL
CSB-MP742573SSL
CSB-EP742573SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP742574SSL
CSB-EP742574SSL
CSB-BP742574SSL
CSB-MP742574SSL
CSB-EP742574SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 50S ribosomal protein L11 (rplK) CSB-YP742575SSL
CSB-EP742575SSL
CSB-BP742575SSL
CSB-MP742575SSL
CSB-EP742575SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Arginine--tRNA ligase (argS), partial CSB-YP742576SSL
CSB-EP742576SSL
CSB-BP742576SSL
CSB-MP742576SSL
CSB-EP742576SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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