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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YLR365W (YLR365W) CSB-YP741976SVG
CSB-EP741976SVG
CSB-BP741976SVG
CSB-MP741976SVG
CSB-EP741976SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Adducin-related protein C1289.14 (SPBC1289.14, SPBC8E4.10c) CSB-YP741977SXV
CSB-EP741977SXV
CSB-BP741977SXV
CSB-MP741977SXV
CSB-EP741977SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable U2 small nuclear ribonucleoprotein B' (pi056, SPBC8D2.09c) CSB-YP741978SXV
CSB-EP741978SXV
CSB-BP741978SXV
CSB-MP741978SXV
CSB-EP741978SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Deinagkistrodon acutus Coagulation factor X inhibitor CSB-YP741979DIX
CSB-EP741979DIX
CSB-BP741979DIX
CSB-MP741979DIX
CSB-EP741979DIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelophylax nigromaculatus Bradykinin-like peptide 3 CSB-YP741980PEM
CSB-EP741980PEM
CSB-BP741980PEM
CSB-MP741980PEM
CSB-EP741980PEM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa sauvagei Phyllocaerulein CSB-YP741981EVA
CSB-EP741981EVA
CSB-BP741981EVA
CSB-MP741981EVA
CSB-EP741981EVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naja kaouthia Phospholipase A2 inhibitor 31 kDa subunit CSB-YP741982NAF
CSB-EP741982NAF
CSB-BP741982NAF
CSB-MP741982NAF
CSB-EP741982NAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Echis pyramidum leakeyi Disintegrin echistatin-gamma CSB-YP741983EAL
CSB-EP741983EAL
CSB-BP741983EAL
CSB-MP741983EAL
CSB-EP741983EAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Protease inhibitor long epsilon-dendrotoxin Arg55 CSB-YP741984DBG
CSB-EP741984DBG
CSB-BP741984DBG
CSB-MP741984DBG
CSB-EP741984DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gloydius ussuriensis Disintegrin ussuristatin-2 CSB-YP741985GGS
CSB-EP741985GGS
CSB-BP741985GGS
CSB-MP741985GGS
CSB-EP741985GGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 16S rRNA (guanine (1405)-N (7))-methyltransferase (sgm) CSB-YP741986MFAH
CSB-EP741986MFAH
CSB-BP741986MFAH
CSB-MP741986MFAH
CSB-EP741986MFAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aesculus hippocastanum Defensin-like protein 1 CSB-YP741987AFAF
CSB-EP741987AFAF
CSB-BP741987AFAF
CSB-MP741987AFAF
CSB-EP741987AFAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gastroclonium coulteri R-phycoerythrin gamma-1 chain, chloroplastic CSB-YP741988GBAZ
CSB-EP741988GBAZ
CSB-BP741988GBAZ
CSB-MP741988GBAZ
CSB-EP741988GBAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chalinolobus morio Hemoglobin subunit alpha (HBA) CSB-YP741989CHM
CSB-EP741989CHM
CSB-BP741989CHM
CSB-MP741989CHM
CSB-EP741989CHM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hapalemur griseus Hemoglobin subunit alpha-1 (HBA1) CSB-YP741990HCF
CSB-EP741990HCF
CSB-BP741990HCF
CSB-MP741990HCF
CSB-EP741990HCF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum RING finger protein B (rngB), partial CSB-YP741991DKK
CSB-EP741991DKK
CSB-BP741991DKK
CSB-MP741991DKK
CSB-EP741991DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamys nipponensis akazara Troponin I CSB-YP741992DSN
CSB-EP741992DSN
CSB-BP741992DSN
CSB-MP741992DSN
CSB-EP741992DSN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligobrachia mashikoi Extracellular giant hemoglobin major globin subunit B2 (ghbB2) CSB-YP741993OAAT
CSB-EP741993OAAT
CSB-BP741993OAAT
CSB-MP741993OAAT
CSB-EP741993OAAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helix aspersa Dart gland peptide CSB-YP741994HVH
CSB-EP741994HVH
CSB-BP741994HVH
CSB-MP741994HVH
CSB-EP741994HVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistocerca gregaria Endocuticle structural glycoprotein SgAbd-9 CSB-YP741995SXO
CSB-EP741995SXO
CSB-BP741995SXO
CSB-MP741995SXO
CSB-EP741995SXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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