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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Vibrio vulnificus UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP742056VCQ
CSB-EP742056VCQ
CSB-BP742056VCQ
CSB-MP742056VCQ
CSB-EP742056VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Phosphoribosylamine--glycine ligase (purD) CSB-YP742057VCQ
CSB-EP742057VCQ
CSB-BP742057VCQ
CSB-MP742057VCQ
CSB-EP742057VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Fumarate reductase subunit D (frdD), partial CSB-YP742058VCQ1
CSB-EP742058VCQ1
CSB-BP742058VCQ1
CSB-MP742058VCQ1
CSB-EP742058VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Putative ribosome biogenesis GTPase RsgA 1 (rsgA1) CSB-YP742059VCQ
CSB-EP742059VCQ
CSB-BP742059VCQ
CSB-MP742059VCQ
CSB-EP742059VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP742060VCQ
CSB-EP742060VCQ
CSB-BP742060VCQ
CSB-MP742060VCQ
CSB-EP742060VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 30S ribosomal protein S12 (rpsL) CSB-YP742061VCQ
CSB-EP742061VCQ
CSB-BP742061VCQ
CSB-MP742061VCQ
CSB-EP742061VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus ATP-dependent protease subunit HslV (hslV) CSB-YP742062VCQ
CSB-EP742062VCQ
CSB-BP742062VCQ
CSB-MP742062VCQ
CSB-EP742062VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Argininosuccinate synthase (argG) CSB-YP742063VCQ
CSB-EP742063VCQ
CSB-BP742063VCQ
CSB-MP742063VCQ
CSB-EP742063VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glucose-6-phosphate isomerase (pgi), partial CSB-YP742064VCQ
CSB-EP742064VCQ
CSB-BP742064VCQ
CSB-MP742064VCQ
CSB-EP742064VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus RNA polymerase-associated protein RapA (rapA), partial CSB-YP742065VCQ
CSB-EP742065VCQ
CSB-BP742065VCQ
CSB-MP742065VCQ
CSB-EP742065VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP742066VCQ
CSB-EP742066VCQ
CSB-BP742066VCQ
CSB-MP742066VCQ
CSB-EP742066VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP742067VCQ
CSB-EP742067VCQ
CSB-BP742067VCQ
CSB-MP742067VCQ
CSB-EP742067VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus DNA repair protein recO (recO) CSB-YP742068VCQ
CSB-EP742068VCQ
CSB-BP742068VCQ
CSB-MP742068VCQ
CSB-EP742068VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Cell division protein FtsB (ftsB), partial CSB-YP742069VCQ
CSB-EP742069VCQ
CSB-BP742069VCQ
CSB-MP742069VCQ
CSB-EP742069VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Protein RecA (recA) CSB-YP742070VCQ
CSB-EP742070VCQ
CSB-BP742070VCQ
CSB-MP742070VCQ
CSB-EP742070VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus 50S ribosomal protein L19 (rplS) CSB-YP742071VCQ
CSB-EP742071VCQ
CSB-BP742071VCQ
CSB-MP742071VCQ
CSB-EP742071VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP742072VCQ
CSB-EP742072VCQ
CSB-BP742072VCQ
CSB-MP742072VCQ
CSB-EP742072VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus tRNA pseudouridine synthase B (truB) CSB-YP742073VCQ
CSB-EP742073VCQ
CSB-BP742073VCQ
CSB-MP742073VCQ
CSB-EP742073VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Elongation factor G 2 (fusA2), partial CSB-YP742074VCQ
CSB-EP742074VCQ
CSB-BP742074VCQ
CSB-MP742074VCQ
CSB-EP742074VCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio vulnificus Na (+)-translocating NADH-quinone reductase subunit D (nqrD), partial CSB-YP742075VCQ1
CSB-EP742075VCQ1
CSB-BP742075VCQ1
CSB-MP742075VCQ1
CSB-EP742075VCQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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