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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP742176PIJ
CSB-EP742176PIJ
CSB-BP742176PIJ
CSB-MP742176PIJ
CSB-EP742176PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP742177PIJ
CSB-EP742177PIJ
CSB-BP742177PIJ
CSB-MP742177PIJ
CSB-EP742177PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP742178PIJ
CSB-EP742178PIJ
CSB-BP742178PIJ
CSB-MP742178PIJ
CSB-EP742178PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosome-binding factor A (rbfA) CSB-YP742179PIJ
CSB-EP742179PIJ
CSB-BP742179PIJ
CSB-MP742179PIJ
CSB-EP742179PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Valine--tRNA ligase (valS), partial CSB-YP742180PIJ
CSB-EP742180PIJ
CSB-BP742180PIJ
CSB-MP742180PIJ
CSB-EP742180PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP742181PIJ
CSB-EP742181PIJ
CSB-BP742181PIJ
CSB-MP742181PIJ
CSB-EP742181PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP742182PIJ
CSB-EP742182PIJ
CSB-BP742182PIJ
CSB-MP742182PIJ
CSB-EP742182PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP742183PIJ
CSB-EP742183PIJ
CSB-BP742183PIJ
CSB-MP742183PIJ
CSB-EP742183PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Uncharacterized protein YjeA (yjeA) CSB-YP742184PIJ
CSB-EP742184PIJ
CSB-BP742184PIJ
CSB-MP742184PIJ
CSB-EP742184PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0042 nucleotide-binding protein plu4044 (plu4044) CSB-YP742185PIJ
CSB-EP742185PIJ
CSB-BP742185PIJ
CSB-MP742185PIJ
CSB-EP742185PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Serine/threonine transporter sstT (sstT), partial CSB-YP742186PIJ
CSB-EP742186PIJ
CSB-BP742186PIJ
CSB-MP742186PIJ
CSB-EP742186PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Cell division protein FtsP (ftsP) CSB-YP742187PIJ
CSB-EP742187PIJ
CSB-BP742187PIJ
CSB-MP742187PIJ
CSB-EP742187PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP742188PIJ
CSB-EP742188PIJ
CSB-BP742188PIJ
CSB-MP742188PIJ
CSB-EP742188PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 7-cyano-7-deazaguanine synthase (queC) CSB-YP742189PIJ
CSB-EP742189PIJ
CSB-BP742189PIJ
CSB-MP742189PIJ
CSB-EP742189PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UDP-2,3-diacylglucosamine hydrolase (lpxH) CSB-YP742190PIJ
CSB-EP742190PIJ
CSB-BP742190PIJ
CSB-MP742190PIJ
CSB-EP742190PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP742191PIJ
CSB-EP742191PIJ
CSB-BP742191PIJ
CSB-MP742191PIJ
CSB-EP742191PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP742192PIJ
CSB-EP742192PIJ
CSB-BP742192PIJ
CSB-MP742192PIJ
CSB-EP742192PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Protein translocase subunit SecA (secA), partial CSB-YP742193PIJ
CSB-EP742193PIJ
CSB-BP742193PIJ
CSB-MP742193PIJ
CSB-EP742193PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Antitoxin HicB 1 (hicB1) CSB-YP742194PIJ
CSB-EP742194PIJ
CSB-BP742194PIJ
CSB-MP742194PIJ
CSB-EP742194PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Elongation factor 4 (lepA), partial CSB-YP742195PIJ
CSB-EP742195PIJ
CSB-BP742195PIJ
CSB-MP742195PIJ
CSB-EP742195PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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