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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Onion yellows phytoplasma UPF0176 protein PAM_085 (PAM_085) CSB-YP744358OBN
CSB-EP744358OBN
CSB-BP744358OBN
CSB-MP744358OBN
CSB-EP744358OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Peptide chain release factor 1 (prfA) CSB-YP744359OBN
CSB-EP744359OBN
CSB-BP744359OBN
CSB-MP744359OBN
CSB-EP744359OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica UPF0392 protein Os08g0121900 (Os08g0121900, LOC_Os08g02850), partial CSB-YP744360OFG1
CSB-EP744360OFG1
CSB-BP744360OFG1
CSB-MP744360OFG1
CSB-EP744360OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CASP-like protein Os07g0442900 (Os07g0442900, LOC_Os07g26110), partial CSB-YP744361OFG1
CSB-EP744361OFG1
CSB-BP744361OFG1
CSB-MP744361OFG1
CSB-EP744361OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Molybdopterin synthase sulfur carrier subunit (Os02g0558300, LOC_Os02g35200) CSB-YP744362OFG
CSB-EP744362OFG
CSB-BP744362OFG
CSB-MP744362OFG
CSB-EP744362OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica CASP-like protein Os02g0219900 (Os02g0219900, LOC_Os02g12760), partial CSB-YP744363OFG1
CSB-EP744363OFG1
CSB-BP744363OFG1
CSB-MP744363OFG1
CSB-EP744363OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cyclin-D4-1 (CYCD4-1) CSB-YP744364OFG
CSB-EP744364OFG
CSB-BP744364OFG
CSB-MP744364OFG
CSB-EP744364OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP744365EVD1
CSB-EP744365EVD1
CSB-BP744365EVD1
CSB-MP744365EVD1
CSB-EP744365EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Translation initiation factor IF-1, chloroplastic (infA) CSB-YP744366EVD
CSB-EP744366EVD
CSB-BP744366EVD
CSB-MP744366EVD
CSB-EP744366EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Photosystem II reaction center protein J (psbJ), partial CSB-YP744367EVD
CSB-EP744367EVD
CSB-BP744367EVD
CSB-MP744367EVD
CSB-EP744367EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 30S ribosomal protein S18, chloroplastic (rps18) CSB-YP744368EVD
CSB-EP744368EVD
CSB-BP744368EVD
CSB-MP744368EVD
CSB-EP744368EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP744369EVD1
CSB-EP744369EVD1
CSB-BP744369EVD1
CSB-MP744369EVD1
CSB-EP744369EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Photosystem II D2 protein (psbD), partial CSB-YP744370EVD1
CSB-EP744370EVD1
CSB-BP744370EVD1
CSB-MP744370EVD1
CSB-EP744370EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens 50S ribosomal protein L21, chloroplastic (rpl21) CSB-YP744371EVD
CSB-EP744371EVD
CSB-BP744371EVD
CSB-MP744371EVD
CSB-EP744371EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Chloroplast envelope membrane protein (cemA), partial CSB-YP744372EVD1
CSB-EP744372EVD1
CSB-BP744372EVD1
CSB-MP744372EVD1
CSB-EP744372EVD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Germin-like protein 8-8 (Os08g0189700, LOC_Os08g09020) CSB-YP744373OFG
CSB-EP744373OFG
CSB-BP744373OFG
CSB-MP744373OFG
CSB-EP744373OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Cation-chloride cotransporter 1 (CCC1), partial CSB-YP744374OFG
CSB-EP744374OFG
CSB-BP744374OFG
CSB-MP744374OFG
CSB-EP744374OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Homeobox-leucine zipper protein HOX20 (HOX20) CSB-YP744375OFG
CSB-EP744375OFG
CSB-BP744375OFG
CSB-MP744375OFG
CSB-EP744375OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Aquaporin NIP2-1 (NIP2-1), partial CSB-YP744376OFG1
CSB-EP744376OFG1
CSB-BP744376OFG1
CSB-MP744376OFG1
CSB-EP744376OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Molybdopterin synthase catalytic subunit (MOCS2) CSB-YP744377OFG
CSB-EP744377OFG
CSB-BP744377OFG
CSB-MP744377OFG
CSB-EP744377OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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