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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. NAD (P)H-quinone oxidoreductase subunit M (ndhM) CSB-YP742577SSL
CSB-EP742577SSL
CSB-BP742577SSL
CSB-MP742577SSL
CSB-EP742577SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP742578SSL
CSB-EP742578SSL
CSB-BP742578SSL
CSB-MP742578SSL
CSB-EP742578SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S7 (rpsG) CSB-YP742579SSL
CSB-EP742579SSL
CSB-BP742579SSL
CSB-MP742579SSL
CSB-EP742579SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S9 (rpsI) CSB-YP742580SSL
CSB-EP742580SSL
CSB-BP742580SSL
CSB-MP742580SSL
CSB-EP742580SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S5 (rpsE) CSB-YP742581SSL
CSB-EP742581SSL
CSB-BP742581SSL
CSB-MP742581SSL
CSB-EP742581SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 30S ribosomal protein S17 (rpsQ) CSB-YP742582SSL
CSB-EP742582SSL
CSB-BP742582SSL
CSB-MP742582SSL
CSB-EP742582SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP742583SSL
CSB-EP742583SSL
CSB-BP742583SSL
CSB-MP742583SSL
CSB-EP742583SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II reaction center protein T (psbT), partial CSB-YP742584SSL
CSB-EP742584SSL
CSB-BP742584SSL
CSB-MP742584SSL
CSB-EP742584SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP742585SSL
CSB-EP742585SSL
CSB-BP742585SSL
CSB-MP742585SSL
CSB-EP742585SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Serine--tRNA ligase (serS) CSB-YP742586SSL
CSB-EP742586SSL
CSB-BP742586SSL
CSB-MP742586SSL
CSB-EP742586SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Apocytochrome f (petA), partial CSB-YP742587SSL1
CSB-EP742587SSL1
CSB-BP742587SSL1
CSB-MP742587SSL1
CSB-EP742587SSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP742588SSL
CSB-EP742588SSL
CSB-BP742588SSL
CSB-MP742588SSL
CSB-EP742588SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Uridylate kinase (pyrH) CSB-YP742589SSL
CSB-EP742589SSL
CSB-BP742589SSL
CSB-MP742589SSL
CSB-EP742589SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP742590SSL
CSB-EP742590SSL
CSB-BP742590SSL
CSB-MP742590SSL
CSB-EP742590SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Imidazole glycerol phosphate synthase subunit hisF1 (hisF1) CSB-YP742591SSL
CSB-EP742591SSL
CSB-BP742591SSL
CSB-MP742591SSL
CSB-EP742591SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Apolipoprotein N-acyltransferase (lnt), partial CSB-YP742592SSL1
CSB-EP742592SSL1
CSB-BP742592SSL1
CSB-MP742592SSL1
CSB-EP742592SSL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Dihydroorotase (pyrC) CSB-YP742593SSL
CSB-EP742593SSL
CSB-BP742593SSL
CSB-MP742593SSL
CSB-EP742593SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Threonine--tRNA ligase (thrS), partial CSB-YP742594SSL
CSB-EP742594SSL
CSB-BP742594SSL
CSB-MP742594SSL
CSB-EP742594SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP742595SSL
CSB-EP742595SSL
CSB-BP742595SSL
CSB-MP742595SSL
CSB-EP742595SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Glucose-6-phosphate isomerase (pgi), partial CSB-YP742596SSL
CSB-EP742596SSL
CSB-BP742596SSL
CSB-MP742596SSL
CSB-EP742596SSL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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