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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopirellula baltica Inosine-5'-monophosphate dehydrogenase (guaB), partial CSB-YP742637RDR
CSB-EP742637RDR
CSB-BP742637RDR
CSB-MP742637RDR
CSB-EP742637RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Tryptophan synthase beta chain (trpB) CSB-YP742638RDR
CSB-EP742638RDR
CSB-BP742638RDR
CSB-MP742638RDR
CSB-EP742638RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0061 protein RB9953 (RB9953), partial CSB-YP742639RDR
CSB-EP742639RDR
CSB-BP742639RDR
CSB-MP742639RDR
CSB-EP742639RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glutamyl-tRNA reductase (hemA) CSB-YP742640RDR
CSB-EP742640RDR
CSB-BP742640RDR
CSB-MP742640RDR
CSB-EP742640RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Peptide chain release factor 1 (prfA) CSB-YP742641RDR
CSB-EP742641RDR
CSB-BP742641RDR
CSB-MP742641RDR
CSB-EP742641RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica UPF0102 protein RB9115 (RB9115) CSB-YP742642RDR
CSB-EP742642RDR
CSB-BP742642RDR
CSB-MP742642RDR
CSB-EP742642RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 10 kDa chaperonin 1 (groS1) CSB-YP742643RDR
CSB-EP742643RDR
CSB-BP742643RDR
CSB-MP742643RDR
CSB-EP742643RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Elongation factor Tu (tuf) CSB-YP742644RDR
CSB-EP742644RDR
CSB-BP742644RDR
CSB-MP742644RDR
CSB-EP742644RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 30S ribosomal protein S8 (rpsH) CSB-YP742645RDR
CSB-EP742645RDR
CSB-BP742645RDR
CSB-MP742645RDR
CSB-EP742645RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L16 (rplP) CSB-YP742646RDR
CSB-EP742646RDR
CSB-BP742646RDR
CSB-MP742646RDR
CSB-EP742646RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L2 (rplB) CSB-YP742647RDR
CSB-EP742647RDR
CSB-BP742647RDR
CSB-MP742647RDR
CSB-EP742647RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP742648RDR
CSB-EP742648RDR
CSB-BP742648RDR
CSB-MP742648RDR
CSB-EP742648RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP742649RDR
CSB-EP742649RDR
CSB-BP742649RDR
CSB-MP742649RDR
CSB-EP742649RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Quinolinate synthase A (nadA) CSB-YP742650RDR
CSB-EP742650RDR
CSB-BP742650RDR
CSB-MP742650RDR
CSB-EP742650RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP742651RDR
CSB-EP742651RDR
CSB-BP742651RDR
CSB-MP742651RDR
CSB-EP742651RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Ribose-phosphate pyrophosphokinase (prs) CSB-YP742652RDR
CSB-EP742652RDR
CSB-BP742652RDR
CSB-MP742652RDR
CSB-EP742652RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Glycogen synthase (glgA), partial CSB-YP742653RDR
CSB-EP742653RDR
CSB-BP742653RDR
CSB-MP742653RDR
CSB-EP742653RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica 50S ribosomal protein L36 (rpmJ) CSB-YP742654RDR
CSB-EP742654RDR
CSB-BP742654RDR
CSB-MP742654RDR
CSB-EP742654RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Adenylyl-sulfate kinase (cysC) CSB-YP742655RDR
CSB-EP742655RDR
CSB-BP742655RDR
CSB-MP742655RDR
CSB-EP742655RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Flagellar assembly factor FliW (fliW) CSB-YP742656RDR
CSB-EP742656RDR
CSB-BP742656RDR
CSB-MP742656RDR
CSB-EP742656RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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