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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus subsp. pastoris 30S ribosomal protein S18 (rpsR) CSB-YP742537EYQ
CSB-EP742537EYQ
CSB-BP742537EYQ
CSB-MP742537EYQ
CSB-EP742537EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus subsp. pastoris Photosystem II reaction center protein M (psbM), partial CSB-YP742538EYQ
CSB-EP742538EYQ
CSB-BP742538EYQ
CSB-MP742538EYQ
CSB-EP742538EYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Isoleucine--tRNA ligase (ileS), partial CSB-YP742539EYO
CSB-EP742539EYO
CSB-BP742539EYO
CSB-MP742539EYO
CSB-EP742539EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus GTPase obg (obg) CSB-YP742540EYO
CSB-EP742540EYO
CSB-BP742540EYO
CSB-MP742540EYO
CSB-EP742540EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP742541EYO
CSB-EP742541EYO
CSB-BP742541EYO
CSB-MP742541EYO
CSB-EP742541EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 30S ribosomal protein S21 (rpsU) CSB-YP742542EYO
CSB-EP742542EYO
CSB-BP742542EYO
CSB-MP742542EYO
CSB-EP742542EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP742543EYO
CSB-EP742543EYO
CSB-BP742543EYO
CSB-MP742543EYO
CSB-EP742543EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA dimethylallyltransferase (miaA) CSB-YP742544EYO
CSB-EP742544EYO
CSB-BP742544EYO
CSB-MP742544EYO
CSB-EP742544EYO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase (fabZ) CSB-YP742545EYP
CSB-EP742545EYP
CSB-BP742545EYP
CSB-MP742545EYP
CSB-EP742545EYP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prephenate dehydratase (pheA) CSB-YP742546MVH
CSB-EP742546MVH
CSB-BP742546MVH
CSB-MP742546MVH
CSB-EP742546MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative S-adenosyl-L-methionine-dependent methyltransferase Mb3793c (Mb3793c) CSB-YP742547MVH
CSB-EP742547MVH
CSB-BP742547MVH
CSB-MP742547MVH
CSB-EP742547MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Long-chain-fatty-acid--CoA ligase FadD17 (fadD17), partial CSB-YP742548MVH
CSB-EP742548MVH
CSB-BP742548MVH
CSB-MP742548MVH
CSB-EP742548MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative thiosulfate sulfurtransferase sseA (sseA) CSB-YP742549MVH
CSB-EP742549MVH
CSB-BP742549MVH
CSB-MP742549MVH
CSB-EP742549MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0182 protein Mb3215c (Mb3215c), partial CSB-YP742550MVH
CSB-EP742550MVH
CSB-BP742550MVH
CSB-MP742550MVH
CSB-EP742550MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP742551MVH
CSB-EP742551MVH
CSB-BP742551MVH
CSB-MP742551MVH
CSB-EP742551MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase (Mb2976) CSB-YP742552MVH
CSB-EP742552MVH
CSB-BP742552MVH
CSB-MP742552MVH
CSB-EP742552MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phthiocerol/phenolphthiocerol synthesis polyketide synthase type I PpsC (ppsC), partial CSB-YP742553MVH
CSB-EP742553MVH
CSB-BP742553MVH
CSB-MP742553MVH
CSB-EP742553MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline--tRNA ligase (proS), partial CSB-YP742554MVH
CSB-EP742554MVH
CSB-BP742554MVH
CSB-MP742554MVH
CSB-EP742554MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP742555MVH
CSB-EP742555MVH
CSB-BP742555MVH
CSB-MP742555MVH
CSB-EP742555MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease Z (rnz) CSB-YP742556MVH
CSB-EP742556MVH
CSB-BP742556MVH
CSB-MP742556MVH
CSB-EP742556MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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