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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella baltica Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146STL
CSB-EP019146STL
CSB-BP019146STL
CSB-MP019146STL
CSB-EP019146STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Serine hydroxymethyltransferase (glyA) CSB-YP021274STL
CSB-EP021274STL
CSB-BP021274STL
CSB-MP021274STL
CSB-EP021274STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica ATP synthase subunit b (atpF), partial CSB-YP002358STL1
CSB-EP002358STL1
CSB-BP002358STL1
CSB-MP002358STL1
CSB-EP002358STL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Argininosuccinate synthase (argG) CSB-YP002234DUT
CSB-EP002234DUT
CSB-BP002234DUT
CSB-MP002234DUT
CSB-EP002234DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DUT
CSB-EP014799DUT
CSB-BP014799DUT
CSB-MP014799DUT
CSB-EP014799DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658DUT
CSB-EP001658DUT
CSB-BP001658DUT
CSB-MP001658DUT
CSB-EP001658DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146DUT
CSB-EP019146DUT
CSB-BP019146DUT
CSB-MP019146DUT
CSB-EP019146DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosome-recycling factor (frr) CSB-YP014941DUT
CSB-EP014941DUT
CSB-BP014941DUT
CSB-MP014941DUT
CSB-EP014941DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Porphobilinogen deaminase (hemC) CSB-YP010524DUT
CSB-EP010524DUT
CSB-BP010524DUT
CSB-MP010524DUT
CSB-EP010524DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taenia asiatica 60S ribosomal protein L18 (RPL18) CSB-YP020154TJX
CSB-EP020154TJX
CSB-BP020154TJX
CSB-MP020154TJX
CSB-EP020154TJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptosporidium parvum Flap endonuclease 1 (FEN1) CSB-YP008585CXO
CSB-EP008585CXO
CSB-BP008585CXO
CSB-MP008585CXO
CSB-EP008585CXO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis mRNA cap guanine-N7 methyltransferase (ABD1), partial CSB-YP019902SXM
CSB-EP019902SXM
CSB-BP019902SXM
CSB-MP019902SXM
CSB-EP019902SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mediator of RNA polymerase II transcription subunit 12 (SRB8), partial CSB-YP013643SXM
CSB-EP013643SXM
CSB-BP013643SXM
CSB-MP013643SXM
CSB-EP013643SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) CSB-YP002426SXM
CSB-EP002426SXM
CSB-BP002426SXM
CSB-MP002426SXM
CSB-EP002426SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mediator of RNA polymerase II transcription subunit 20 (SRB2) CSB-YP013653SXM
CSB-EP013653SXM
CSB-BP013653SXM
CSB-MP013653SXM
CSB-EP013653SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H3.3 (HHT3) CSB-YP010109SXM
CSB-EP010109SXM
CSB-BP010109SXM
CSB-MP010109SXM
CSB-EP010109SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Ubiquitin-like protein ATG12 (ATG12) CSB-YP002283SXM
CSB-EP002283SXM
CSB-BP002283SXM
CSB-MP002283SXM
CSB-EP002283SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Filamin A-interacting protein 1-like (filip1l), partial CSB-YP008680DIL
CSB-EP008680DIL
CSB-BP008680DIL
CSB-MP008680DIL
CSB-EP008680DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein canopy homolog 3 (cnpy3) CSB-YP005676DIL
CSB-EP005676DIL
CSB-BP005676DIL
CSB-MP005676DIL
CSB-EP005676DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Mediator of RNA polymerase II transcription subunit 13 (SSN2), partial CSB-YP013645SXM
CSB-EP013645SXM
CSB-BP013645SXM
CSB-MP013645SXM
CSB-EP013645SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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