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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylibium petroleiphilum Nucleoside diphosphate kinase (ndk) CSB-YP015889MNX
CSB-EP015889MNX
CSB-BP015889MNX
CSB-MP015889MNX
CSB-EP015889MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylibium petroleiphilum Malate dehydrogenase (mdh) CSB-YP013623MNX
CSB-EP013623MNX
CSB-BP013623MNX
CSB-MP013623MNX
CSB-EP013623MNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Flap endonuclease 1 (fen) CSB-YP008585MNT
CSB-EP008585MNT
CSB-BP008585MNT
CSB-MP008585MNT
CSB-EP008585MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Diphthine synthase (dphB) CSB-YP007133MNT
CSB-EP007133MNT
CSB-BP007133MNT
CSB-MP007133MNT
CSB-EP007133MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Nucleoside diphosphate kinase (ndk) CSB-YP015889MNT
CSB-EP015889MNT
CSB-BP015889MNT
CSB-MP015889MNT
CSB-EP015889MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein spinster homolog 2 (spns2), partial CSB-YP022592DIL
CSB-EP022592DIL
CSB-BP022592DIL
CSB-MP022592DIL
CSB-EP022592DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca nemestrina Homeobox protein Hox-C10 (HOXC10) CSB-YP010671MOX
CSB-EP010671MOX
CSB-BP010671MOX
CSB-MP010671MOX
CSB-EP010671MOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Zinc finger and SCAN domain-containing protein 12 (ZSCAN12), partial CSB-YP027132EQV
CSB-EP027132EQV
CSB-BP027132EQV
CSB-MP027132EQV
CSB-EP027132EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Homeobox protein Hox-D8 (HOXD8) CSB-YP010688EQV
CSB-EP010688EQV
CSB-BP010688EQV
CSB-MP010688EQV
CSB-EP010688EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Putative homeobox protein NANOG2 (NANOGP1) CSB-YP015425EQV
CSB-EP015425EQV
CSB-BP015425EQV
CSB-MP015425EQV
CSB-EP015425EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Homeobox protein MOX-1 (MEOX1) CSB-YP013695EQV
CSB-EP013695EQV
CSB-BP013695EQV
CSB-MP013695EQV
CSB-EP013695EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Zinc finger and BTB domain-containing protein 32 (ZBTB32) CSB-YP026325EQV
CSB-EP026325EQV
CSB-BP026325EQV
CSB-MP026325EQV
CSB-EP026325EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Homeobox protein Hox-A7 (HOXA7) CSB-YP010657EQV
CSB-EP010657EQV
CSB-BP010657EQV
CSB-MP010657EQV
CSB-EP010657EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Oxidoreductase HTATIP2 (HTATIP2) CSB-YP010871EXP
CSB-EP010871EXP
CSB-BP010871EXP
CSB-MP010871EXP
CSB-EP010871EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Homeobox protein goosecoid (GSC) CSB-YP009950EXP
CSB-EP009950EXP
CSB-BP009950EXP
CSB-MP009950EXP
CSB-EP009950EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Polyglutamine-binding protein 1 (PQBP1) CSB-YP018594EXP
CSB-EP018594EXP
CSB-BP018594EXP
CSB-MP018594EXP
CSB-EP018594EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Post-GPI attachment to proteins factor 3 (PGAP3), partial CSB-YP017789DXU1
CSB-EP017789DXU1
CSB-BP017789DXU1
CSB-MP017789DXU1
CSB-EP017789DXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cyclic AMP-responsive element-binding protein 3-like protein 2 (creb3l2), partial CSB-YP005950XBE1
CSB-EP005950XBE1
CSB-BP005950XBE1
CSB-MP005950XBE1
CSB-EP005950XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein kish-A (tmem167a), partial CSB-YP023740XBE
CSB-EP023740XBE
CSB-BP023740XBE
CSB-MP023740XBE
CSB-EP023740XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Alcohol dehydrogenase class-3 (ADHIII) CSB-YP001357OFF
CSB-EP001357OFF
CSB-BP001357OFF
CSB-MP001357OFF
CSB-EP001357OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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