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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Gamma-hemolysin component B (hlgB) CSB-YP743237SKW
CSB-EP743237SKW
CSB-BP743237SKW
CSB-MP743237SKW
CSB-EP743237SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine protease splF (splF) CSB-YP743238SKW
CSB-EP743238SKW
CSB-BP743238SKW
CSB-MP743238SKW
CSB-EP743238SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator rot (rot) CSB-YP743239SKW
CSB-EP743239SKW
CSB-BP743239SKW
CSB-MP743239SKW
CSB-EP743239SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP743240SKW
CSB-EP743240SKW
CSB-BP743240SKW
CSB-MP743240SKW
CSB-EP743240SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP743241SKW
CSB-EP743241SKW
CSB-BP743241SKW
CSB-MP743241SKW
CSB-EP743241SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative universal stress protein SAS1637 (SAS1637) CSB-YP743242SKW
CSB-EP743242SKW
CSB-BP743242SKW
CSB-MP743242SKW
CSB-EP743242SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP743243SKW
CSB-EP743243SKW
CSB-BP743243SKW
CSB-MP743243SKW
CSB-EP743243SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Trigger factor (tig) CSB-YP743244SKW
CSB-EP743244SKW
CSB-BP743244SKW
CSB-MP743244SKW
CSB-EP743244SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Valine--tRNA ligase (valS), partial CSB-YP743245SKW
CSB-EP743245SKW
CSB-BP743245SKW
CSB-MP743245SKW
CSB-EP743245SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP743246SKW
CSB-EP743246SKW
CSB-BP743246SKW
CSB-MP743246SKW
CSB-EP743246SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Queuine tRNA-ribosyltransferase (tgt) CSB-YP743247SKW
CSB-EP743247SKW
CSB-BP743247SKW
CSB-MP743247SKW
CSB-EP743247SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP743248SKW
CSB-EP743248SKW
CSB-BP743248SKW
CSB-MP743248SKW
CSB-EP743248SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription elongation factor GreA (greA) CSB-YP743249SKW
CSB-EP743249SKW
CSB-BP743249SKW
CSB-MP743249SKW
CSB-EP743249SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP743250SKW
CSB-EP743250SKW
CSB-BP743250SKW
CSB-MP743250SKW
CSB-EP743250SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chaperone protein DnaK (dnaK), partial CSB-YP743251SKW
CSB-EP743251SKW
CSB-BP743251SKW
CSB-MP743251SKW
CSB-EP743251SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable endonuclease 4 (nfo) CSB-YP743252SKW
CSB-EP743252SKW
CSB-BP743252SKW
CSB-MP743252SKW
CSB-EP743252SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP743253SKW
CSB-EP743253SKW
CSB-BP743253SKW
CSB-MP743253SKW
CSB-EP743253SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Cytidylate kinase (cmk) CSB-YP743254SKW
CSB-EP743254SKW
CSB-BP743254SKW
CSB-MP743254SKW
CSB-EP743254SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Demethylmenaquinone methyltransferase (ubiE) CSB-YP743255SKW
CSB-EP743255SKW
CSB-BP743255SKW
CSB-MP743255SKW
CSB-EP743255SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus CCA-adding enzyme (cca) CSB-YP743256SKW
CSB-EP743256SKW
CSB-BP743256SKW
CSB-MP743256SKW
CSB-EP743256SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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