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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Cell cycle protein GpsB (gpsB) CSB-YP743257SKW
CSB-EP743257SKW
CSB-BP743257SKW
CSB-MP743257SKW
CSB-EP743257SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0403 protein SAS1371 (SAS1371) CSB-YP743258SKW
CSB-EP743258SKW
CSB-BP743258SKW
CSB-MP743258SKW
CSB-EP743258SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide methionine sulfoxide reductase MsrA 2 (msrA2) CSB-YP743259SKW
CSB-EP743259SKW
CSB-BP743259SKW
CSB-MP743259SKW
CSB-EP743259SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable CtpA-like serine protease (SAS1363), partial CSB-YP743260SKW1
CSB-EP743260SKW1
CSB-BP743260SKW1
CSB-MP743260SKW1
CSB-EP743260SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acylphosphatase (acyP) CSB-YP743261SKW
CSB-EP743261SKW
CSB-BP743261SKW
CSB-MP743261SKW
CSB-EP743261SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphate-binding protein pstS (pstS) CSB-YP743262SKW
CSB-EP743262SKW
CSB-BP743262SKW
CSB-MP743262SKW
CSB-EP743262SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP743263SKW
CSB-EP743263SKW
CSB-BP743263SKW
CSB-MP743263SKW
CSB-EP743263SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAS1292 (SAS1292) CSB-YP743264SKW
CSB-EP743264SKW
CSB-BP743264SKW
CSB-MP743264SKW
CSB-EP743264SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 2 (rpmG2) CSB-YP743265SKW
CSB-EP743265SKW
CSB-BP743265SKW
CSB-MP743265SKW
CSB-EP743265SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycerol kinase (glpK) CSB-YP743266SKW
CSB-EP743266SKW
CSB-BP743266SKW
CSB-MP743266SKW
CSB-EP743266SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA translocase FtsK (ftsK), partial CSB-YP743267SKW1
CSB-EP743267SKW1
CSB-BP743267SKW1
CSB-MP743267SKW1
CSB-EP743267SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosome maturation factor RimP (rimP) CSB-YP743268SKW
CSB-EP743268SKW
CSB-BP743268SKW
CSB-MP743268SKW
CSB-EP743268SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uridylate kinase (pyrH) CSB-YP743269SKW
CSB-EP743269SKW
CSB-BP743269SKW
CSB-MP743269SKW
CSB-EP743269SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP743270SKW
CSB-EP743270SKW
CSB-BP743270SKW
CSB-MP743270SKW
CSB-EP743270SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP743271SKW
CSB-EP743271SKW
CSB-BP743271SKW
CSB-MP743271SKW
CSB-EP743271SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Methionyl-tRNA formyltransferase (fmt) CSB-YP743272SKW
CSB-EP743272SKW
CSB-BP743272SKW
CSB-MP743272SKW
CSB-EP743272SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Laccase domain protein SAS1121 (SAS1121) CSB-YP743273SKW
CSB-EP743273SKW
CSB-BP743273SKW
CSB-MP743273SKW
CSB-EP743273SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Carbamate kinase 1 (arcC1) CSB-YP743274SKW
CSB-EP743274SKW
CSB-BP743274SKW
CSB-MP743274SKW
CSB-EP743274SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UvrABC system protein C (uvrC), partial CSB-YP743275SKW
CSB-EP743275SKW
CSB-BP743275SKW
CSB-MP743275SKW
CSB-EP743275SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable heme-iron transport system permease protein IsdF (isdF), partial CSB-YP743276SKW
CSB-EP743276SKW
CSB-BP743276SKW
CSB-MP743276SKW
CSB-EP743276SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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