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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella henselae 50S ribosomal protein L35 (rpmI) CSB-YP743197BSG
CSB-EP743197BSG
CSB-BP743197BSG
CSB-MP743197BSG
CSB-EP743197BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 30S ribosomal protein S15 (rpsO) CSB-YP743198BSG
CSB-EP743198BSG
CSB-BP743198BSG
CSB-MP743198BSG
CSB-EP743198BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae sn-glycerol-3-phosphate import ATP-binding protein UgpC (ugpC) CSB-YP743199BSG
CSB-EP743199BSG
CSB-BP743199BSG
CSB-MP743199BSG
CSB-EP743199BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae ATP synthase subunit b 2 (atpF2), partial CSB-YP743200BSG
CSB-EP743200BSG
CSB-BP743200BSG
CSB-MP743200BSG
CSB-EP743200BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae UPF0176 protein BH12760 (BH12760) CSB-YP743201BSG
CSB-EP743201BSG
CSB-BP743201BSG
CSB-MP743201BSG
CSB-EP743201BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Protein TolB (tolB) CSB-YP743202BSG
CSB-EP743202BSG
CSB-BP743202BSG
CSB-MP743202BSG
CSB-EP743202BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Protease HtpX homolog (htpX), partial CSB-YP743203BSG1
CSB-EP743203BSG1
CSB-BP743203BSG1
CSB-MP743203BSG1
CSB-EP743203BSG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lactonase drp35 (drp35) CSB-YP743204SKW
CSB-EP743204SKW
CSB-BP743204SKW
CSB-MP743204SKW
CSB-EP743204SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP743205SKW
CSB-EP743205SKW
CSB-BP743205SKW
CSB-MP743205SKW
CSB-EP743205SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Capsular polysaccharide biosynthesis protein CapA (capA), partial CSB-YP743206SKW1
CSB-EP743206SKW1
CSB-BP743206SKW1
CSB-MP743206SKW1
CSB-EP743206SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized hydrolase SAS2467 (SAS2467) CSB-YP743207SKW
CSB-EP743207SKW
CSB-BP743207SKW
CSB-MP743207SKW
CSB-EP743207SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Diapolycopene oxygenase (crtP) CSB-YP743208SKW
CSB-EP743208SKW
CSB-BP743208SKW
CSB-MP743208SKW
CSB-EP743208SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Copper-exporting P-type ATPase A (copA), partial CSB-YP743209SKW
CSB-EP743209SKW
CSB-BP743209SKW
CSB-MP743209SKW
CSB-EP743209SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holin-like protein CidA (cidA), partial CSB-YP743210SKW1
CSB-EP743210SKW1
CSB-BP743210SKW1
CSB-MP743210SKW1
CSB-EP743210SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Gamma-hemolysin component A (hlgA) CSB-YP743211SKW
CSB-EP743211SKW
CSB-BP743211SKW
CSB-MP743211SKW
CSB-EP743211SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme sensor protein hssS (hssS), partial CSB-YP743212SKW1
CSB-EP743212SKW1
CSB-BP743212SKW1
CSB-MP743212SKW1
CSB-EP743212SKW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Lysostaphin resistance protein A (lyrA), partial CSB-YP743213SKW
CSB-EP743213SKW
CSB-BP743213SKW
CSB-MP743213SKW
CSB-EP743213SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative 2-hydroxyacid dehydrogenase SAS2196 (SAS2196) CSB-YP743214SKW
CSB-EP743214SKW
CSB-BP743214SKW
CSB-MP743214SKW
CSB-EP743214SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit gamma (ureA) CSB-YP743215SKW
CSB-EP743215SKW
CSB-BP743215SKW
CSB-MP743215SKW
CSB-EP743215SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized HTH-type transcriptional regulator SAS2155 (SAS2155) CSB-YP743216SKW
CSB-EP743216SKW
CSB-BP743216SKW
CSB-MP743216SKW
CSB-EP743216SKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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