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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermotoga petrophila tRNA dimethylallyltransferase (miaA) CSB-YP024529TKF
CSB-EP024529TKF
CSB-BP024529TKF
CSB-MP024529TKF
CSB-EP024529TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila ATP synthase subunit b (atpF), partial CSB-YP002358TKF1
CSB-EP002358TKF1
CSB-BP002358TKF1
CSB-MP002358TKF1
CSB-EP002358TKF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga petrophila Galactokinase (galK) CSB-YP009198TKF
CSB-EP009198TKF
CSB-BP009198TKF
CSB-MP009198TKF
CSB-EP009198TKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycine cleavage system H protein (gcvH) CSB-YP009335SUJ
CSB-EP009335SUJ
CSB-BP009335SUJ
CSB-MP009335SUJ
CSB-EP009335SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Era (era) CSB-YP007759SUJ
CSB-EP007759SUJ
CSB-BP007759SUJ
CSB-MP007759SUJ
CSB-EP007759SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Porphobilinogen deaminase (hemC) CSB-YP010524SUJ
CSB-EP010524SUJ
CSB-BP010524SUJ
CSB-MP010524SUJ
CSB-EP010524SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit beta (atpD) CSB-YP002350SUJ
CSB-EP002350SUJ
CSB-BP002350SUJ
CSB-MP002350SUJ
CSB-EP002350SUJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit a (atpB), partial CSB-YP015070SUJ1
CSB-EP015070SUJ1
CSB-BP015070SUJ1
CSB-MP015070SUJ1
CSB-EP015070SUJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae ATP synthase subunit delta (atpH) CSB-YP002355MWC
CSB-EP002355MWC
CSB-BP002355MWC
CSB-MP002355MWC
CSB-EP002355MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Triosephosphate isomerase (tpiA) CSB-YP024102MWC
CSB-EP024102MWC
CSB-BP024102MWC
CSB-MP024102MWC
CSB-EP024102MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma agalactiae Peptide chain release factor 1 (prfA) CSB-YP015197MWC
CSB-EP015197MWC
CSB-BP015197MWC
CSB-MP015197MWC
CSB-EP015197MWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vacuolar ATPase assembly integral membrane protein VMA21 (pdcd-2), partial CSB-YP025866CXY1
CSB-EP025866CXY1
CSB-BP025866CXY1
CSB-MP025866CXY1
CSB-EP025866CXY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Elongation factor Tu (tuf1) CSB-YP025342CWX
CSB-EP025342CWX
CSB-BP025342CWX
CSB-MP025342CWX
CSB-EP025342CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium kluyveri Serine hydroxymethyltransferase (glyA) CSB-YP021274CWX
CSB-EP021274CWX
CSB-BP021274CWX
CSB-MP021274CWX
CSB-EP021274CWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium johnsoniae ATP synthase subunit b (atpF), partial CSB-YP002358FDT1
CSB-EP002358FDT1
CSB-BP002358FDT1
CSB-MP002358FDT1
CSB-EP002358FDT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Thymidylate kinase (tmk) CSB-YP007226DHU
CSB-EP007226DHU
CSB-BP007226DHU
CSB-MP007226DHU
CSB-EP007226DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Triosephosphate isomerase (tpiA) CSB-YP024102DHU
CSB-EP024102DHU
CSB-BP024102DHU
CSB-MP024102DHU
CSB-EP024102DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dehalococcoides sp. Serine hydroxymethyltransferase (glyA) CSB-YP021274DHU
CSB-EP021274DHU
CSB-BP021274DHU
CSB-MP021274DHU
CSB-EP021274DHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Glycine cleavage system H protein (gcvH) CSB-YP009335ATZ
CSB-EP009335ATZ
CSB-BP009335ATZ
CSB-MP009335ATZ
CSB-EP009335ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidiphilium cryptum Translation initiation factor IF-2 (infB), partial CSB-YP015164ATZ
CSB-EP015164ATZ
CSB-BP015164ATZ
CSB-MP015164ATZ
CSB-EP015164ATZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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