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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermus thermophilus UPF0447 protein TT_C1352 (TT_C1352) CSB-YP744618TNS
CSB-EP744618TNS
CSB-BP744618TNS
CSB-MP744618TNS
CSB-EP744618TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L23 (rplW) CSB-YP744619TNS
CSB-EP744619TNS
CSB-BP744619TNS
CSB-MP744619TNS
CSB-EP744619TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L5 (rplE) CSB-YP744620TNS
CSB-EP744620TNS
CSB-BP744620TNS
CSB-MP744620TNS
CSB-EP744620TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 50S ribosomal protein L36 (rpmJ) CSB-YP744621TNS
CSB-EP744621TNS
CSB-BP744621TNS
CSB-MP744621TNS
CSB-EP744621TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Redox-sensing transcriptional repressor rex (rex) CSB-YP744622TNS
CSB-EP744622TNS
CSB-BP744622TNS
CSB-MP744622TNS
CSB-EP744622TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus UPF0747 protein TT_C1201 (TT_C1201) CSB-YP744623TNS
CSB-EP744623TNS
CSB-BP744623TNS
CSB-MP744623TNS
CSB-EP744623TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus tRNA (Ile)-lysidine synthase (tilS), partial CSB-YP744624TNS
CSB-EP744624TNS
CSB-BP744624TNS
CSB-MP744624TNS
CSB-EP744624TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Glutamyl-tRNA reductase (hemA) CSB-YP744625TNS
CSB-EP744625TNS
CSB-BP744625TNS
CSB-MP744625TNS
CSB-EP744625TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Shikimate kinase (aroK) CSB-YP744626TNS
CSB-EP744626TNS
CSB-BP744626TNS
CSB-MP744626TNS
CSB-EP744626TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 3-dehydroquinate dehydratase (aroQ) CSB-YP744627TNS
CSB-EP744627TNS
CSB-BP744627TNS
CSB-MP744627TNS
CSB-EP744627TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus DNA mismatch repair protein mutL (mutL), partial CSB-YP744628TNS
CSB-EP744628TNS
CSB-BP744628TNS
CSB-MP744628TNS
CSB-EP744628TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP744629TNS
CSB-EP744629TNS
CSB-BP744629TNS
CSB-MP744629TNS
CSB-EP744629TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Ketol-acid reductoisomerase (ilvC) CSB-YP744630TNS
CSB-EP744630TNS
CSB-BP744630TNS
CSB-MP744630TNS
CSB-EP744630TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Elongation factor P (efp) CSB-YP744631TNS
CSB-EP744631TNS
CSB-BP744631TNS
CSB-MP744631TNS
CSB-EP744631TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus DNA ligase (ligA), partial CSB-YP744632TNS
CSB-EP744632TNS
CSB-BP744632TNS
CSB-MP744632TNS
CSB-EP744632TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Shikimate dehydrogenase (aroE) CSB-YP744633TNS
CSB-EP744633TNS
CSB-BP744633TNS
CSB-MP744633TNS
CSB-EP744633TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Quinolinate synthase A (nadA) CSB-YP744634TNS
CSB-EP744634TNS
CSB-BP744634TNS
CSB-MP744634TNS
CSB-EP744634TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus tRNA modification GTPase MnmE (mnmE) CSB-YP744635TNS
CSB-EP744635TNS
CSB-BP744635TNS
CSB-MP744635TNS
CSB-EP744635TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Elongation factor Ts (tsf) CSB-YP744636TNS
CSB-EP744636TNS
CSB-BP744636TNS
CSB-MP744636TNS
CSB-EP744636TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Translation initiation factor IF-2 (infB), partial CSB-YP744637TNS
CSB-EP744637TNS
CSB-BP744637TNS
CSB-MP744637TNS
CSB-EP744637TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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