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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant tRNA (cytidine (34)-2'-O)-methyltransferase (trmL) CSB-YP744939YAS
CSB-EP744939YAS
CSB-BP744939YAS
CSB-MP744939YAS
CSB-EP744939YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii H/ACA ribonucleoprotein complex subunit 3 (NOP10) CSB-YP744940DOT
CSB-EP744940DOT
CSB-BP744940DOT
CSB-MP744940DOT
CSB-EP744940DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 5'-3' exoribonuclease 2 (RAT1), partial CSB-YP744941DOT
CSB-EP744941DOT
CSB-BP744941DOT
CSB-MP744941DOT
CSB-EP744941DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein SEY1 (SEY1), partial CSB-YP744942DOT1
CSB-EP744942DOT1
CSB-BP744942DOT1
CSB-MP744942DOT1
CSB-EP744942DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nuclear protein localization protein 4 (NPL4), partial CSB-YP744943DOT
CSB-EP744943DOT
CSB-BP744943DOT
CSB-MP744943DOT
CSB-EP744943DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA (guanine-N (7)-)-methyltransferase (TRM8) CSB-YP744944DOT
CSB-EP744944DOT
CSB-BP744944DOT
CSB-MP744944DOT
CSB-EP744944DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor SLU7 (SLU7) CSB-YP744945DOT
CSB-EP744945DOT
CSB-BP744945DOT
CSB-MP744945DOT
CSB-EP744945DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable metalloprotease ARX1 (ARX1), partial CSB-YP744946DOT
CSB-EP744946DOT
CSB-BP744946DOT
CSB-MP744946DOT
CSB-EP744946DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pentafunctional AROM polypeptide (ARO1), partial CSB-YP744947DOT
CSB-EP744947DOT
CSB-BP744947DOT
CSB-MP744947DOT
CSB-EP744947DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype C Protein Vpu (vpu), partial CSB-YP744948HDAH
CSB-EP744948HDAH
CSB-BP744948HDAH
CSB-MP744948HDAH
CSB-EP744948HDAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine palmitoyltransferase-regulating protein TSC3 (TSC3), partial CSB-YP744949DOT1
CSB-EP744949DOT1
CSB-BP744949DOT1
CSB-MP744949DOT1
CSB-EP744949DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Type 1 phosphatases regulator YPI1 (YPI1) CSB-YP744950DOT
CSB-EP744950DOT
CSB-BP744950DOT
CSB-MP744950DOT
CSB-EP744950DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor SLT11 (SLT11) CSB-YP744951DOT
CSB-EP744951DOT
CSB-BP744951DOT
CSB-MP744951DOT
CSB-EP744951DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GPN-loop GTPase 3 homolog AGL117C (AGL117C) CSB-YP744952DOT
CSB-EP744952DOT
CSB-BP744952DOT
CSB-MP744952DOT
CSB-EP744952DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDH4), partial CSB-YP744953DOT1
CSB-EP744953DOT1
CSB-BP744953DOT1
CSB-MP744953DOT1
CSB-EP744953DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Acyl-protein thioesterase 1 (AGL188W) CSB-YP744954DOT
CSB-EP744954DOT
CSB-BP744954DOT
CSB-MP744954DOT
CSB-EP744954DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP6 (DBP6), partial CSB-YP744955DOT
CSB-EP744955DOT
CSB-BP744955DOT
CSB-MP744955DOT
CSB-EP744955DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Elongin-C (ELC1) CSB-YP744956DOT
CSB-EP744956DOT
CSB-BP744956DOT
CSB-MP744956DOT
CSB-EP744956DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Dol-P-Man:Man (5)GlcNAc (2)-PP-Dol alpha-1,3-mannosyltransferase (ALG3), partial CSB-YP744957DOT
CSB-EP744957DOT
CSB-BP744957DOT
CSB-MP744957DOT
CSB-EP744957DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Iron-sulfur clusters transporter ATM1, mitochondrial (ATM1), partial CSB-YP744958DOT1
CSB-EP744958DOT1
CSB-BP744958DOT1
CSB-MP744958DOT1
CSB-EP744958DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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