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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anopheles gambiae Probable methylthioribulose-1-phosphate dehydratase (AGAP000470) CSB-YP745742BZL
CSB-EP745742BZL
CSB-BP745742BZL
CSB-MP745742BZL
CSB-EP745742BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Mediator of RNA polymerase II transcription subunit 29 (ix) CSB-YP745743BZL
CSB-EP745743BZL
CSB-BP745743BZL
CSB-MP745743BZL
CSB-EP745743BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Probable cytosolic Fe-S cluster assembly factor AGAP009023 (AGAP009023) CSB-YP745744BZL
CSB-EP745744BZL
CSB-BP745744BZL
CSB-MP745744BZL
CSB-EP745744BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Histone deacetylase complex subunit SAP30 homolog (AGAP001654) CSB-YP745745BZL
CSB-EP745745BZL
CSB-BP745745BZL
CSB-MP745745BZL
CSB-EP745745BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae UPF0518 protein AGAP011705 (AGAP011705), partial CSB-YP745746BZL
CSB-EP745746BZL
CSB-BP745746BZL
CSB-MP745746BZL
CSB-EP745746BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Eukaryotic translation initiation factor 3 subunit M (AGAP012281) CSB-YP745747BZL
CSB-EP745747BZL
CSB-BP745747BZL
CSB-MP745747BZL
CSB-EP745747BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Histone-arginine methyltransferase CARMER (CARM1), partial CSB-YP745748BZL
CSB-EP745748BZL
CSB-BP745748BZL
CSB-MP745748BZL
CSB-EP745748BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Inosine triphosphate pyrophosphatase (AGAP008374) CSB-YP745749BZL
CSB-EP745749BZL
CSB-BP745749BZL
CSB-MP745749BZL
CSB-EP745749BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae tRNA (guanine (37)-N1)-methyltransferase (AGAP006124), partial CSB-YP745750BZL
CSB-EP745750BZL
CSB-BP745750BZL
CSB-MP745750BZL
CSB-EP745750BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Queuine tRNA-ribosyltransferase subunit QTRTD1 homolog (AGAP005542) CSB-YP745751BZL
CSB-EP745751BZL
CSB-BP745751BZL
CSB-MP745751BZL
CSB-EP745751BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae S-methyl-5'-thioadenosine phosphorylase (AGAP005129) CSB-YP745752BZL
CSB-EP745752BZL
CSB-BP745752BZL
CSB-MP745752BZL
CSB-EP745752BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Eukaryotic translation initiation factor 3 subunit F (eIF3-S5) CSB-YP745753BZL
CSB-EP745753BZL
CSB-BP745753BZL
CSB-MP745753BZL
CSB-EP745753BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Glutamyl-tRNA (Gln) amidotransferase subunit A, mitochondrial (gatA) CSB-YP745754BZL
CSB-EP745754BZL
CSB-BP745754BZL
CSB-MP745754BZL
CSB-EP745754BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae 60S ribosomal protein L18 (RpL18) CSB-YP745755BZL
CSB-EP745755BZL
CSB-BP745755BZL
CSB-MP745755BZL
CSB-EP745755BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial (PY05764), partial CSB-YP745756PMT
CSB-EP745756PMT
CSB-BP745756PMT
CSB-MP745756PMT
CSB-EP745756PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anamorsin homolog (PY03124) CSB-YP745757PMT
CSB-EP745757PMT
CSB-BP745757PMT
CSB-MP745757PMT
CSB-EP745757PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flap endonuclease 1 (FEN1) CSB-YP745758PMT
CSB-EP745758PMT
CSB-BP745758PMT
CSB-MP745758PMT
CSB-EP745758PMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human STE20-related kinase adapter protein alpha (STRADA) CSB-YP745759HU
CSB-EP745759HU
CSB-BP745759HU
CSB-MP745759HU
CSB-EP745759HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Otopetrin-3 (OTOP3), partial CSB-YP745760HU
CSB-EP745760HU
CSB-BP745760HU
CSB-MP745760HU
CSB-EP745760HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Equilibrative nucleoside transporter 4 (SLC29A4), partial CSB-YP745761HU
CSB-EP745761HU
CSB-BP745761HU
CSB-MP745761HU
CSB-EP745761HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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