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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Putative formate dehydrogenase MW2229 (MW2229), partial CSB-YP745139SUL
CSB-EP745139SUL
CSB-BP745139SUL
CSB-MP745139SUL
CSB-EP745139SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L14 (rplN) CSB-YP745140SUL
CSB-EP745140SUL
CSB-BP745140SUL
CSB-MP745140SUL
CSB-EP745140SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus PTS system lactose-specific EIICB component (lacE), partial CSB-YP745141SUL
CSB-EP745141SUL
CSB-BP745141SUL
CSB-MP745141SUL
CSB-EP745141SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable DEAD-box ATP-dependent RNA helicase MW2004 (MW2004), partial CSB-YP745142SUL
CSB-EP745142SUL
CSB-BP745142SUL
CSB-MP745142SUL
CSB-EP745142SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Low molecular weight protein-tyrosine-phosphatase ptpA (ptpA) CSB-YP745143SUL
CSB-EP745143SUL
CSB-BP745143SUL
CSB-MP745143SUL
CSB-EP745143SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator rot (rot) CSB-YP745144SUL
CSB-EP745144SUL
CSB-BP745144SUL
CSB-MP745144SUL
CSB-EP745144SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable cell wall amidase LytH (lytH) CSB-YP745145SUL
CSB-EP745145SUL
CSB-BP745145SUL
CSB-MP745145SUL
CSB-EP745145SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0403 protein MW1467 (MW1467) CSB-YP745146SUL
CSB-EP745146SUL
CSB-BP745146SUL
CSB-MP745146SUL
CSB-EP745146SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0403 protein MW1318 (MW1318) CSB-YP745147SUL
CSB-EP745147SUL
CSB-BP745147SUL
CSB-MP745147SUL
CSB-EP745147SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport system permease protein oppC2 (oppC2), partial CSB-YP745148SUL1
CSB-EP745148SUL1
CSB-BP745148SUL1
CSB-MP745148SUL1
CSB-EP745148SUL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S15 (rpsO) CSB-YP745149SUL
CSB-EP745149SUL
CSB-BP745149SUL
CSB-MP745149SUL
CSB-EP745149SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus High-affinity heme uptake system protein isdE (isdE) CSB-YP745150SUL
CSB-EP745150SUL
CSB-BP745150SUL
CSB-MP745150SUL
CSB-EP745150SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease J 1 (MW0972), partial CSB-YP745151SUL
CSB-EP745151SUL
CSB-BP745151SUL
CSB-MP745151SUL
CSB-EP745151SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0477 protein MW0896 (MW0896) CSB-YP745152SUL
CSB-EP745152SUL
CSB-BP745152SUL
CSB-MP745152SUL
CSB-EP745152SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0051 protein MW0799 (MW0799) CSB-YP745153SUL
CSB-EP745153SUL
CSB-BP745153SUL
CSB-MP745153SUL
CSB-EP745153SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP745154SUL
CSB-EP745154SUL
CSB-BP745154SUL
CSB-MP745154SUL
CSB-EP745154SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Response regulator protein graR (graR) CSB-YP745155SUL
CSB-EP745155SUL
CSB-BP745155SUL
CSB-MP745155SUL
CSB-EP745155SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative acetyl-CoA C-acetyltransferase vraB (vraB) CSB-YP745156SUL
CSB-EP745156SUL
CSB-BP745156SUL
CSB-MP745156SUL
CSB-EP745156SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP745157SUL
CSB-EP745157SUL
CSB-BP745157SUL
CSB-MP745157SUL
CSB-EP745157SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Formate acetyltransferase (pflB), partial CSB-YP745158SUL
CSB-EP745158SUL
CSB-BP745158SUL
CSB-MP745158SUL
CSB-EP745158SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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