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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bartonella henselae Aspartate carbamoyltransferase (pyrB) CSB-YP746542BSG
CSB-EP746542BSG
CSB-BP746542BSG
CSB-MP746542BSG
CSB-EP746542BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 50S ribosomal protein L1 (rplA) CSB-YP746543BSG
CSB-EP746543BSG
CSB-BP746543BSG
CSB-MP746543BSG
CSB-EP746543BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Trigger factor (tig) CSB-YP746544BSG
CSB-EP746544BSG
CSB-BP746544BSG
CSB-MP746544BSG
CSB-EP746544BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 50S ribosomal protein L9 (rplI) CSB-YP746545BSG
CSB-EP746545BSG
CSB-BP746545BSG
CSB-MP746545BSG
CSB-EP746545BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Dihydrodipicolinate synthase (dapA) CSB-YP746546BSG
CSB-EP746546BSG
CSB-BP746546BSG
CSB-MP746546BSG
CSB-EP746546BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP746547BSG
CSB-EP746547BSG
CSB-BP746547BSG
CSB-MP746547BSG
CSB-EP746547BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP746548BSG
CSB-EP746548BSG
CSB-BP746548BSG
CSB-MP746548BSG
CSB-EP746548BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Putative ABC transporter ATP-binding protein BH02760 (BH02760) CSB-YP746549BSG
CSB-EP746549BSG
CSB-BP746549BSG
CSB-MP746549BSG
CSB-EP746549BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Isoleucine--tRNA ligase (ileS), partial CSB-YP746550BSG
CSB-EP746550BSG
CSB-BP746550BSG
CSB-MP746550BSG
CSB-EP746550BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP746551BSG
CSB-EP746551BSG
CSB-BP746551BSG
CSB-MP746551BSG
CSB-EP746551BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP746552BSG
CSB-EP746552BSG
CSB-BP746552BSG
CSB-MP746552BSG
CSB-EP746552BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Glycerol-3-phosphate dehydrogenase [NAD (P)+] (gpsA) CSB-YP746553BSG
CSB-EP746553BSG
CSB-BP746553BSG
CSB-MP746553BSG
CSB-EP746553BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Protein-export protein SecB (secB) CSB-YP746554BSG
CSB-EP746554BSG
CSB-BP746554BSG
CSB-MP746554BSG
CSB-EP746554BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae tRNA pseudouridine synthase A (truA) CSB-YP746555BSG
CSB-EP746555BSG
CSB-BP746555BSG
CSB-MP746555BSG
CSB-EP746555BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Protein grpE (grpE) CSB-YP746556BSG
CSB-EP746556BSG
CSB-BP746556BSG
CSB-MP746556BSG
CSB-EP746556BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Maf-like protein BH00020 (BH00020) CSB-YP746557BSG
CSB-EP746557BSG
CSB-BP746557BSG
CSB-MP746557BSG
CSB-EP746557BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 50S ribosomal protein L20 (rplT) CSB-YP746558BSG
CSB-EP746558BSG
CSB-BP746558BSG
CSB-MP746558BSG
CSB-EP746558BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP746559BSG
CSB-EP746559BSG
CSB-BP746559BSG
CSB-MP746559BSG
CSB-EP746559BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae Cysteine--tRNA ligase (cysS), partial CSB-YP746560BSG
CSB-EP746560BSG
CSB-BP746560BSG
CSB-MP746560BSG
CSB-EP746560BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella henselae 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP746561BSG
CSB-EP746561BSG
CSB-BP746561BSG
CSB-MP746561BSG
CSB-EP746561BSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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