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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus johnsonii Ribonuclease 3 (rnc) CSB-YP744899LAW
CSB-EP744899LAW
CSB-BP744899LAW
CSB-MP744899LAW
CSB-EP744899LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Undecaprenyl pyrophosphate synthase (uppS) CSB-YP744900LAW
CSB-EP744900LAW
CSB-BP744900LAW
CSB-MP744900LAW
CSB-EP744900LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii 30S ribosomal protein S21 (rpsU) CSB-YP744901LAW
CSB-EP744901LAW
CSB-BP744901LAW
CSB-MP744901LAW
CSB-EP744901LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Dihydroorotate dehydrogenase A (fumarate) (pyrD) CSB-YP744902LAW
CSB-EP744902LAW
CSB-BP744902LAW
CSB-MP744902LAW
CSB-EP744902LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Asparagine--tRNA ligase (asnS) CSB-YP744903LAW
CSB-EP744903LAW
CSB-BP744903LAW
CSB-MP744903LAW
CSB-EP744903LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Ribonuclease HII (rnhB) CSB-YP744904LAW
CSB-EP744904LAW
CSB-BP744904LAW
CSB-MP744904LAW
CSB-EP744904LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii UPF0346 protein LJ_1103 (LJ_1103) CSB-YP744905LAW
CSB-EP744905LAW
CSB-BP744905LAW
CSB-MP744905LAW
CSB-EP744905LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Segregation and condensation protein A (scpA) CSB-YP744906LAW
CSB-EP744906LAW
CSB-BP744906LAW
CSB-MP744906LAW
CSB-EP744906LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP744907LAW
CSB-EP744907LAW
CSB-BP744907LAW
CSB-MP744907LAW
CSB-EP744907LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Protein MraZ (mraZ) CSB-YP744908LAW
CSB-EP744908LAW
CSB-BP744908LAW
CSB-MP744908LAW
CSB-EP744908LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Probable tRNA sulfurtransferase (thiI) CSB-YP744909LAW
CSB-EP744909LAW
CSB-BP744909LAW
CSB-MP744909LAW
CSB-EP744909LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii ATP synthase epsilon chain (atpC) CSB-YP744910LAW
CSB-EP744910LAW
CSB-BP744910LAW
CSB-MP744910LAW
CSB-EP744910LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii ATP synthase subunit alpha (atpA), partial CSB-YP744911LAW
CSB-EP744911LAW
CSB-BP744911LAW
CSB-MP744911LAW
CSB-EP744911LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Thymidine kinase (tdk) CSB-YP744912LAW
CSB-EP744912LAW
CSB-BP744912LAW
CSB-MP744912LAW
CSB-EP744912LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Triosephosphate isomerase (tpiA) CSB-YP744913LAW
CSB-EP744913LAW
CSB-BP744913LAW
CSB-MP744913LAW
CSB-EP744913LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Ribose-5-phosphate isomerase A (rpiA) CSB-YP744914LAW
CSB-EP744914LAW
CSB-BP744914LAW
CSB-MP744914LAW
CSB-EP744914LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Non-canonical purine NTP pyrophosphatase (LJ_0483) CSB-YP744915LAW
CSB-EP744915LAW
CSB-BP744915LAW
CSB-MP744915LAW
CSB-EP744915LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii DNA mismatch repair protein mutL (mutL), partial CSB-YP744916LAW
CSB-EP744916LAW
CSB-BP744916LAW
CSB-MP744916LAW
CSB-EP744916LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii UPF0133 protein LJ_0424 (LJ_0424) CSB-YP744917LAW
CSB-EP744917LAW
CSB-BP744917LAW
CSB-MP744917LAW
CSB-EP744917LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus johnsonii Protein translocase subunit SecA 2 (secA2), partial CSB-YP744918LAW
CSB-EP744918LAW
CSB-BP744918LAW
CSB-MP744918LAW
CSB-EP744918LAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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