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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii UPF0758 protein plu4865 (plu4865) CSB-YP745522PIJ
CSB-EP745522PIJ
CSB-BP745522PIJ
CSB-MP745522PIJ
CSB-EP745522PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii D,D-heptose 1,7-bisphosphate phosphatase 2 (gmhB2) CSB-YP745523PIJ
CSB-EP745523PIJ
CSB-BP745523PIJ
CSB-MP745523PIJ
CSB-EP745523PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii ATP-dependent protease subunit HslV (hslV) CSB-YP745524PIJ
CSB-EP745524PIJ
CSB-BP745524PIJ
CSB-MP745524PIJ
CSB-EP745524PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Pantothenate kinase (coaA) CSB-YP745525PIJ
CSB-EP745525PIJ
CSB-BP745525PIJ
CSB-MP745525PIJ
CSB-EP745525PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S3 (rpsC) CSB-YP745526PIJ
CSB-EP745526PIJ
CSB-BP745526PIJ
CSB-MP745526PIJ
CSB-EP745526PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S8 (rpsH) CSB-YP745527PIJ
CSB-EP745527PIJ
CSB-BP745527PIJ
CSB-MP745527PIJ
CSB-EP745527PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP745528PIJ
CSB-EP745528PIJ
CSB-BP745528PIJ
CSB-MP745528PIJ
CSB-EP745528PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ketol-acid reductoisomerase (ilvC) CSB-YP745529PIJ
CSB-EP745529PIJ
CSB-BP745529PIJ
CSB-MP745529PIJ
CSB-EP745529PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Magnesium transport protein CorA (corA), partial CSB-YP745530PIJ1
CSB-EP745530PIJ1
CSB-BP745530PIJ1
CSB-MP745530PIJ1
CSB-EP745530PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP745531PIJ
CSB-EP745531PIJ
CSB-BP745531PIJ
CSB-MP745531PIJ
CSB-EP745531PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 50S ribosomal protein L21 (rplU) CSB-YP745532PIJ
CSB-EP745532PIJ
CSB-BP745532PIJ
CSB-MP745532PIJ
CSB-EP745532PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Translation initiation factor IF-2 (infB), partial CSB-YP745533PIJ
CSB-EP745533PIJ
CSB-BP745533PIJ
CSB-MP745533PIJ
CSB-EP745533PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sec-independent protein translocase protein TatB (tatB) CSB-YP745534PIJ
CSB-EP745534PIJ
CSB-BP745534PIJ
CSB-MP745534PIJ
CSB-EP745534PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Chorismate--pyruvate lyase (ubiC) CSB-YP745535PIJ
CSB-EP745535PIJ
CSB-BP745535PIJ
CSB-MP745535PIJ
CSB-EP745535PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Bifunctional protein FolD (folD) CSB-YP745536PIJ
CSB-EP745536PIJ
CSB-BP745536PIJ
CSB-MP745536PIJ
CSB-EP745536PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Sulfurtransferase TusA (tusA) CSB-YP745537PIJ
CSB-EP745537PIJ
CSB-BP745537PIJ
CSB-MP745537PIJ
CSB-EP745537PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii 30S ribosomal protein S9 (rpsI) CSB-YP745538PIJ
CSB-EP745538PIJ
CSB-BP745538PIJ
CSB-MP745538PIJ
CSB-EP745538PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP745539PIJ1
CSB-EP745539PIJ1
CSB-BP745539PIJ1
CSB-MP745539PIJ1
CSB-EP745539PIJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Acyl carrier protein phosphodiesterase (acpH) CSB-YP745540PIJ
CSB-EP745540PIJ
CSB-BP745540PIJ
CSB-MP745540PIJ
CSB-EP745540PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0234 protein plu3881 (plu3881) CSB-YP745541PIJ
CSB-EP745541PIJ
CSB-BP745541PIJ
CSB-MP745541PIJ
CSB-EP745541PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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