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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photorhabdus luminescens subsp. laumondii Phosphate import ATP-binding protein PstB (pstB) CSB-YP745602PIJ
CSB-EP745602PIJ
CSB-BP745602PIJ
CSB-MP745602PIJ
CSB-EP745602PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP745603PIJ
CSB-EP745603PIJ
CSB-BP745603PIJ
CSB-MP745603PIJ
CSB-EP745603PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii UPF0276 protein plu0081 (plu0081) CSB-YP745604PIJ
CSB-EP745604PIJ
CSB-BP745604PIJ
CSB-MP745604PIJ
CSB-EP745604PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP745605PIJ
CSB-EP745605PIJ
CSB-BP745605PIJ
CSB-MP745605PIJ
CSB-EP745605PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photorhabdus luminescens subsp. laumondii Bifunctional protein GlmU (glmU) CSB-YP745606PIJ
CSB-EP745606PIJ
CSB-BP745606PIJ
CSB-MP745606PIJ
CSB-EP745606PIJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Bifunctional protein FolD (folD) CSB-YP745607MWI
CSB-EP745607MWI
CSB-BP745607MWI
CSB-MP745607MWI
CSB-EP745607MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 30S ribosomal protein S18 (rpsR) CSB-YP745608MWI
CSB-EP745608MWI
CSB-BP745608MWI
CSB-MP745608MWI
CSB-EP745608MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) CSB-YP745609MWI
CSB-EP745609MWI
CSB-BP745609MWI
CSB-MP745609MWI
CSB-EP745609MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Cytadherence high molecular weight protein 1 (hlp1), partial CSB-YP745610MWI
CSB-EP745610MWI
CSB-BP745610MWI
CSB-MP745610MWI
CSB-EP745610MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Elongation factor G (fusA), partial CSB-YP745611MWI
CSB-EP745611MWI
CSB-BP745611MWI
CSB-MP745611MWI
CSB-EP745611MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Acetate kinase (ackA) CSB-YP745612MWI
CSB-EP745612MWI
CSB-BP745612MWI
CSB-MP745612MWI
CSB-EP745612MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 50S ribosomal protein L33 1 (rpmG1) CSB-YP745613MWI
CSB-EP745613MWI
CSB-BP745613MWI
CSB-MP745613MWI
CSB-EP745613MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Probable rRNA maturation factor (MYCGA3940) CSB-YP745614MWI
CSB-EP745614MWI
CSB-BP745614MWI
CSB-MP745614MWI
CSB-EP745614MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP745615MWI
CSB-EP745615MWI
CSB-BP745615MWI
CSB-MP745615MWI
CSB-EP745615MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Ferredoxin--NADP reductase (MYCGA3300) CSB-YP745616MWI
CSB-EP745616MWI
CSB-BP745616MWI
CSB-MP745616MWI
CSB-EP745616MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP745617MWI
CSB-EP745617MWI
CSB-BP745617MWI
CSB-MP745617MWI
CSB-EP745617MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum GTPase Der (der) CSB-YP745618MWI
CSB-EP745618MWI
CSB-BP745618MWI
CSB-MP745618MWI
CSB-EP745618MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum 30S ribosomal protein S20 (rpsT) CSB-YP745619MWI
CSB-EP745619MWI
CSB-BP745619MWI
CSB-MP745619MWI
CSB-EP745619MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Uridylate kinase (pyrH) CSB-YP745620MWI
CSB-EP745620MWI
CSB-BP745620MWI
CSB-MP745620MWI
CSB-EP745620MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Arginine--tRNA ligase (argS), partial CSB-YP745621MWI
CSB-EP745621MWI
CSB-BP745621MWI
CSB-MP745621MWI
CSB-EP745621MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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