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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chromobacterium violaceum Malate dehydrogenase (mdh) CSB-YP745722CKA
CSB-EP745722CKA
CSB-BP745722CKA
CSB-MP745722CKA
CSB-EP745722CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP745723CKA
CSB-EP745723CKA
CSB-BP745723CKA
CSB-MP745723CKA
CSB-EP745723CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP745724CKA
CSB-EP745724CKA
CSB-BP745724CKA
CSB-MP745724CKA
CSB-EP745724CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Shikimate kinase (aroK) CSB-YP745725CKA
CSB-EP745725CKA
CSB-BP745725CKA
CSB-MP745725CKA
CSB-EP745725CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP745726CKA
CSB-EP745726CKA
CSB-BP745726CKA
CSB-MP745726CKA
CSB-EP745726CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP745727CKA
CSB-EP745727CKA
CSB-BP745727CKA
CSB-MP745727CKA
CSB-EP745727CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP745728CKA
CSB-EP745728CKA
CSB-BP745728CKA
CSB-MP745728CKA
CSB-EP745728CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP745729CKA
CSB-EP745729CKA
CSB-BP745729CKA
CSB-MP745729CKA
CSB-EP745729CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Leucine--tRNA ligase (leuS), partial CSB-YP745730CKA
CSB-EP745730CKA
CSB-BP745730CKA
CSB-MP745730CKA
CSB-EP745730CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP745731CKA
CSB-EP745731CKA
CSB-BP745731CKA
CSB-MP745731CKA
CSB-EP745731CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Imidazolonepropionase (hutI) CSB-YP745732CKA
CSB-EP745732CKA
CSB-BP745732CKA
CSB-MP745732CKA
CSB-EP745732CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glucose-6-phosphate isomerase 1 (pgi1), partial CSB-YP745733CKA
CSB-EP745733CKA
CSB-BP745733CKA
CSB-MP745733CKA
CSB-EP745733CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromobacterium violaceum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP745734CKA
CSB-EP745734CKA
CSB-BP745734CKA
CSB-MP745734CKA
CSB-EP745734CKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative ecotin-like protein (rsib_orf.1082) CSB-YP745735RND
CSB-EP745735RND
CSB-BP745735RND
CSB-MP745735RND
CSB-EP745735RND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Probable xyloglucan glycosyltransferase 2 (CSLC2), partial CSB-YP745736OFF1
CSB-EP745736OFF1
CSB-BP745736OFF1
CSB-MP745736OFF1
CSB-EP745736OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter G family member 41 (ABCG41), partial CSB-YP745737DOA
CSB-EP745737DOA
CSB-BP745737DOA
CSB-MP745737DOA
CSB-EP745737DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae UPF0443 protein AGAP003534 (AGAP003534), partial CSB-YP745738BZL1
CSB-EP745738BZL1
CSB-BP745738BZL1
CSB-MP745738BZL1
CSB-EP745738BZL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Nipped-B protein (Nipped-B), partial CSB-YP745739DLU
CSB-EP745739DLU
CSB-BP745739DLU
CSB-MP745739DLU
CSB-EP745739DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Coiled-coil domain-containing protein 72 homolog (AGAP008008) CSB-YP745740BZL
CSB-EP745740BZL
CSB-BP745740BZL
CSB-MP745740BZL
CSB-EP745740BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae Coiled-coil domain-containing protein AGAP005037 (AGAP005037), partial CSB-YP745741BZL
CSB-EP745741BZL
CSB-BP745741BZL
CSB-MP745741BZL
CSB-EP745741BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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