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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bordetella pertussis Ribosome maturation factor RimP (rimP) CSB-YP746322BUA
CSB-EP746322BUA
CSB-BP746322BUA
CSB-MP746322BUA
CSB-EP746322BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Lysine--tRNA ligase (lysS), partial CSB-YP746323BUA
CSB-EP746323BUA
CSB-BP746323BUA
CSB-MP746323BUA
CSB-EP746323BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Uracil phosphoribosyltransferase (upp) CSB-YP746324BUA
CSB-EP746324BUA
CSB-BP746324BUA
CSB-MP746324BUA
CSB-EP746324BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Phosphoglycerate kinase (pgk) CSB-YP746325BUA
CSB-EP746325BUA
CSB-BP746325BUA
CSB-MP746325BUA
CSB-EP746325BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP746326BUA
CSB-EP746326BUA
CSB-BP746326BUA
CSB-MP746326BUA
CSB-EP746326BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP746327BUA
CSB-EP746327BUA
CSB-BP746327BUA
CSB-MP746327BUA
CSB-EP746327BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Ketol-acid reductoisomerase (ilvC) CSB-YP746328BUA
CSB-EP746328BUA
CSB-BP746328BUA
CSB-MP746328BUA
CSB-EP746328BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Release factor glutamine methyltransferase (prmC) CSB-YP746329BUA
CSB-EP746329BUA
CSB-BP746329BUA
CSB-MP746329BUA
CSB-EP746329BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Thiamine-phosphate synthase (thiE) CSB-YP746330BUA
CSB-EP746330BUA
CSB-BP746330BUA
CSB-MP746330BUA
CSB-EP746330BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 7-cyano-7-deazaguanine synthase (queC) CSB-YP746331BUA
CSB-EP746331BUA
CSB-BP746331BUA
CSB-MP746331BUA
CSB-EP746331BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis UPF0176 protein BP0179 (BP0179) CSB-YP746332BUA
CSB-EP746332BUA
CSB-BP746332BUA
CSB-MP746332BUA
CSB-EP746332BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Chaperone protein htpG (htpG), partial CSB-YP746333BUA
CSB-EP746333BUA
CSB-BP746333BUA
CSB-MP746333BUA
CSB-EP746333BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis 50S ribosomal protein L11 (rplK) CSB-YP746334BUA
CSB-EP746334BUA
CSB-BP746334BUA
CSB-MP746334BUA
CSB-EP746334BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP746335BIK
CSB-EP746335BIK
CSB-BP746335BIK
CSB-MP746335BIK
CSB-EP746335BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP746336BIK
CSB-EP746336BIK
CSB-BP746336BIK
CSB-MP746336BIK
CSB-EP746336BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine--tRNA ligase (metG), partial CSB-YP746337BIK
CSB-EP746337BIK
CSB-BP746337BIK
CSB-MP746337BIK
CSB-EP746337BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphomethylpyrimidine synthase (thiC), partial CSB-YP746338BIK
CSB-EP746338BIK
CSB-BP746338BIK
CSB-MP746338BIK
CSB-EP746338BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide deformylase 1 (def1) CSB-YP746339BIK
CSB-EP746339BIK
CSB-BP746339BIK
CSB-MP746339BIK
CSB-EP746339BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0250 protein BPP0168 (BPP0168) CSB-YP746340BIK
CSB-EP746340BIK
CSB-BP746340BIK
CSB-MP746340BIK
CSB-EP746340BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable GTP-binding protein EngB (engB) CSB-YP746341BIK
CSB-EP746341BIK
CSB-BP746341BIK
CSB-MP746341BIK
CSB-EP746341BIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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