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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus TelA-like protein SAR1418 (SAR1418) CSB-YP746724SKV
CSB-EP746724SKV
CSB-BP746724SKV
CSB-MP746724SKV
CSB-EP746724SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrodipicolinate reductase (dapB) CSB-YP746725SKV
CSB-EP746725SKV
CSB-BP746725SKV
CSB-MP746725SKV
CSB-EP746725SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative oligopeptide transport system permease protein oppB2 (oppB2), partial CSB-YP746726SKV1
CSB-EP746726SKV1
CSB-BP746726SKV1
CSB-MP746726SKV1
CSB-EP746726SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tryptophan synthase beta chain (trpB) CSB-YP746727SKV
CSB-EP746727SKV
CSB-BP746727SKV
CSB-MP746727SKV
CSB-EP746727SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable tautomerase SAR1376 (SAR1376) CSB-YP746728SKV
CSB-EP746728SKV
CSB-BP746728SKV
CSB-MP746728SKV
CSB-EP746728SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein GlcT (glcT) CSB-YP746729SKV
CSB-EP746729SKV
CSB-BP746729SKV
CSB-MP746729SKV
CSB-EP746729SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uncharacterized protein SAR1364 (SAR1364) CSB-YP746730SKV
CSB-EP746730SKV
CSB-BP746730SKV
CSB-MP746730SKV
CSB-EP746730SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0154 protein SAR1353 (SAR1353), partial CSB-YP746731SKV
CSB-EP746731SKV
CSB-BP746731SKV
CSB-MP746731SKV
CSB-EP746731SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L33 2 (rpmG2) CSB-YP746732SKV
CSB-EP746732SKV
CSB-BP746732SKV
CSB-MP746732SKV
CSB-EP746732SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP746733SKV1
CSB-EP746733SKV1
CSB-BP746733SKV1
CSB-MP746733SKV1
CSB-EP746733SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Proline--tRNA ligase (proS), partial CSB-YP746734SKV
CSB-EP746734SKV
CSB-BP746734SKV
CSB-MP746734SKV
CSB-EP746734SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Undecaprenyl pyrophosphate synthase (uppS) CSB-YP746735SKV
CSB-EP746735SKV
CSB-BP746735SKV
CSB-MP746735SKV
CSB-EP746735SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Tyrosine recombinase XerC (xerC) CSB-YP746736SKV
CSB-EP746736SKV
CSB-BP746736SKV
CSB-MP746736SKV
CSB-EP746736SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S16 (rpsP) CSB-YP746737SKV
CSB-EP746737SKV
CSB-BP746737SKV
CSB-MP746737SKV
CSB-EP746737SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP746738SKV
CSB-EP746738SKV
CSB-BP746738SKV
CSB-MP746738SKV
CSB-EP746738SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Guanylate kinase (gmk) CSB-YP746739SKV
CSB-EP746739SKV
CSB-BP746739SKV
CSB-MP746739SKV
CSB-EP746739SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional protein pyrR (pyrR) CSB-YP746740SKV
CSB-EP746740SKV
CSB-BP746740SKV
CSB-MP746740SKV
CSB-EP746740SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP746741SKV
CSB-EP746741SKV
CSB-BP746741SKV
CSB-MP746741SKV
CSB-EP746741SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Fibrinogen-binding protein (fib) CSB-YP746742SKV
CSB-EP746742SKV
CSB-BP746742SKV
CSB-MP746742SKV
CSB-EP746742SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-regulated surface determinant protein C (isdC) CSB-YP746743SKV
CSB-EP746743SKV
CSB-BP746743SKV
CSB-MP746743SKV
CSB-EP746743SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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