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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus tropicalis Tubulin beta chain (tubb) CSB-YP746784XBF
CSB-EP746784XBF
CSB-BP746784XBF
CSB-MP746784XBF
CSB-EP746784XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Monoacylglycerol lipase abhd6-A (abhd6-a), partial CSB-YP746785XBE
CSB-EP746785XBE
CSB-BP746785XBE
CSB-MP746785XBE
CSB-EP746785XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein RMD5 homolog A (rmnd5a) CSB-YP746786XBE
CSB-EP746786XBE
CSB-BP746786XBE
CSB-MP746786XBE
CSB-EP746786XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis THO complex subunit 4-B (alyref-b) CSB-YP746787XBE
CSB-EP746787XBE
CSB-BP746787XBE
CSB-MP746787XBE
CSB-EP746787XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein (snrnp27) CSB-YP746788XBE
CSB-EP746788XBE
CSB-BP746788XBE
CSB-MP746788XBE
CSB-EP746788XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein slowmo homolog 1 (slmo1) CSB-YP746789XBE
CSB-EP746789XBE
CSB-BP746789XBE
CSB-MP746789XBE
CSB-EP746789XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GTP cyclohydrolase 1 feedback regulatory protein (gchfr) CSB-YP746790XBE
CSB-EP746790XBE
CSB-BP746790XBE
CSB-MP746790XBE
CSB-EP746790XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Inactive rhomboid protein 1 (rhbdf1), partial CSB-YP746791DIL
CSB-EP746791DIL
CSB-BP746791DIL
CSB-MP746791DIL
CSB-EP746791DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Fatty-acid amide hydrolase 2 (FAAH2), partial CSB-YP746792HU1
CSB-EP746792HU1
CSB-BP746792HU1
CSB-MP746792HU1
CSB-EP746792HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Acetyl-CoA acetyltransferase B, mitochondrial (acat1-b) CSB-YP746793XBE
CSB-EP746793XBE
CSB-BP746793XBE
CSB-MP746793XBE
CSB-EP746793XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Trimeric intracellular cation channel type B-B (tmem38b-b), partial CSB-YP746794XBE1
CSB-EP746794XBE1
CSB-BP746794XBE1
CSB-MP746794XBE1
CSB-EP746794XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein disulfide-isomerase TMX3 (tmx3), partial CSB-YP746795XBE
CSB-EP746795XBE
CSB-BP746795XBE
CSB-MP746795XBE
CSB-EP746795XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Eukaryotic translation initiation factor 3 subunit K (eif3k) CSB-YP746796XBE
CSB-EP746796XBE
CSB-BP746796XBE
CSB-MP746796XBE
CSB-EP746796XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ciliogenesis and planar polarity effector 2 (cplane2) CSB-YP746797XBE
CSB-EP746797XBE
CSB-BP746797XBE
CSB-MP746797XBE
CSB-EP746797XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SEC14 domain and spectrin repeat-containing protein 1-B (sestd1-b), partial CSB-YP746798XBE
CSB-EP746798XBE
CSB-BP746798XBE
CSB-MP746798XBE
CSB-EP746798XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cysteine and histidine-rich protein 1-A (cyhr1-a) CSB-YP746799XBE
CSB-EP746799XBE
CSB-BP746799XBE
CSB-MP746799XBE
CSB-EP746799XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis UPF0443 protein C11orf75 homolog, partial CSB-YP746800XBE
CSB-EP746800XBE
CSB-BP746800XBE
CSB-MP746800XBE
CSB-EP746800XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis F-box-like/WD repeat-containing protein TBL1XR1-B (tbl1xr1-b), partial CSB-YP746801XBE
CSB-EP746801XBE
CSB-BP746801XBE
CSB-MP746801XBE
CSB-EP746801XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SHC SH2 domain-binding protein 1 homolog A (shcbp1-a), partial CSB-YP746802XBE
CSB-EP746802XBE
CSB-BP746802XBE
CSB-MP746802XBE
CSB-EP746802XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Growth factor receptor-bound protein 2-B (grb2-b) CSB-YP746803XBE
CSB-EP746803XBE
CSB-BP746803XBE
CSB-MP746803XBE
CSB-EP746803XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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