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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii mRNA 3'-end-processing protein RNA14 (RNA14), partial CSB-YP748384DOT
CSB-EP748384DOT
CSB-BP748384DOT
CSB-MP748384DOT
CSB-EP748384DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Peptidyl-prolyl cis-trans isomerase D (CPR6) CSB-YP748385DOT
CSB-EP748385DOT
CSB-BP748385DOT
CSB-MP748385DOT
CSB-EP748385DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Probable nucleolar complex protein 14 (NOP14), partial CSB-YP748386DOT
CSB-EP748386DOT
CSB-BP748386DOT
CSB-MP748386DOT
CSB-EP748386DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM50 (TIM50), partial CSB-YP748387DOT1
CSB-EP748387DOT1
CSB-BP748387DOT1
CSB-MP748387DOT1
CSB-EP748387DOT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein FMP52, mitochondrial (FMP52) CSB-YP748388DOT
CSB-EP748388DOT
CSB-BP748388DOT
CSB-MP748388DOT
CSB-EP748388DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii NEDD8-conjugating enzyme UBC12 (UBC12) CSB-YP748389DOT
CSB-EP748389DOT
CSB-BP748389DOT
CSB-MP748389DOT
CSB-EP748389DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein DSE1 (DSE1), partial CSB-YP748390DOT
CSB-EP748390DOT
CSB-BP748390DOT
CSB-MP748390DOT
CSB-EP748390DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6) CSB-YP748391DOT
CSB-EP748391DOT
CSB-BP748391DOT
CSB-MP748391DOT
CSB-EP748391DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii KNR4/SMI1 homolog (ADL139W), partial CSB-YP748392DOT
CSB-EP748392DOT
CSB-BP748392DOT
CSB-MP748392DOT
CSB-EP748392DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Polyadenylation factor subunit 2 (PSF2) CSB-YP748393DOT
CSB-EP748393DOT
CSB-BP748393DOT
CSB-MP748393DOT
CSB-EP748393DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RNA polymerase II holoenzyme cyclin-like subunit (SSN8) CSB-YP748394DOT
CSB-EP748394DOT
CSB-BP748394DOT
CSB-MP748394DOT
CSB-EP748394DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Leukotriene A-4 hydrolase homolog (ADL233W), partial CSB-YP748395DOT
CSB-EP748395DOT
CSB-BP748395DOT
CSB-MP748395DOT
CSB-EP748395DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DED1 (DED1), partial CSB-YP748396DOT
CSB-EP748396DOT
CSB-BP748396DOT
CSB-MP748396DOT
CSB-EP748396DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Non-histone chromosomal protein 6 (NHP6) CSB-YP748397DOT
CSB-EP748397DOT
CSB-BP748397DOT
CSB-MP748397DOT
CSB-EP748397DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii L-aminoadipate-semialdehyde dehydrogenase large subunit (LYS2), partial CSB-YP748398DOT
CSB-EP748398DOT
CSB-BP748398DOT
CSB-MP748398DOT
CSB-EP748398DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GPI mannosyltransferase 3 (GPI10), partial CSB-YP748399DOT
CSB-EP748399DOT
CSB-BP748399DOT
CSB-MP748399DOT
CSB-EP748399DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii mRNA-decapping enzyme subunit 2 (DCP2), partial CSB-YP748400DOT
CSB-EP748400DOT
CSB-BP748400DOT
CSB-MP748400DOT
CSB-EP748400DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii U6 snRNA-associated Sm-like protein LSm6 (LSM6) CSB-YP748401DOT
CSB-EP748401DOT
CSB-BP748401DOT
CSB-MP748401DOT
CSB-EP748401DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii PAB-dependent poly (A)-specific ribonuclease subunit PAN3 (PAN3), partial CSB-YP748402DOT
CSB-EP748402DOT
CSB-BP748402DOT
CSB-MP748402DOT
CSB-EP748402DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP748403DOT
CSB-EP748403DOT
CSB-BP748403DOT
CSB-MP748403DOT
CSB-EP748403DOT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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