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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Probable tRNA sulfurtransferase (thiI) CSB-YP746704SKV
CSB-EP746704SKV
CSB-BP746704SKV
CSB-MP746704SKV
CSB-EP746704SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit alpha (dnaE), partial CSB-YP746705SKV
CSB-EP746705SKV
CSB-BP746705SKV
CSB-MP746705SKV
CSB-EP746705SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 50S ribosomal protein L35 (rpmI) CSB-YP746706SKV
CSB-EP746706SKV
CSB-BP746706SKV
CSB-MP746706SKV
CSB-EP746706SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase obg (obg) CSB-YP746707SKV
CSB-EP746707SKV
CSB-BP746707SKV
CSB-MP746707SKV
CSB-EP746707SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Histidine--tRNA ligase (hisS) CSB-YP746708SKV
CSB-EP746708SKV
CSB-BP746708SKV
CSB-MP746708SKV
CSB-EP746708SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative Holliday junction resolvase (SAR1695) CSB-YP746709SKV
CSB-EP746709SKV
CSB-BP746709SKV
CSB-MP746709SKV
CSB-EP746709SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Transcription elongation factor GreA (greA) CSB-YP746710SKV
CSB-EP746710SKV
CSB-BP746710SKV
CSB-MP746710SKV
CSB-EP746710SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0271 protein SAR1684 (SAR1684), partial CSB-YP746711SKV
CSB-EP746711SKV
CSB-BP746711SKV
CSB-MP746711SKV
CSB-EP746711SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heat-inducible transcription repressor HrcA (hrcA) CSB-YP746712SKV
CSB-EP746712SKV
CSB-BP746712SKV
CSB-MP746712SKV
CSB-EP746712SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycine--tRNA ligase (glyQS) CSB-YP746713SKV
CSB-EP746713SKV
CSB-BP746713SKV
CSB-MP746713SKV
CSB-EP746713SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable endonuclease 4 (nfo) CSB-YP746714SKV
CSB-EP746714SKV
CSB-BP746714SKV
CSB-MP746714SKV
CSB-EP746714SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP746715SKV
CSB-EP746715SKV
CSB-BP746715SKV
CSB-MP746715SKV
CSB-EP746715SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP746716SKV
CSB-EP746716SKV
CSB-BP746716SKV
CSB-MP746716SKV
CSB-EP746716SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyrroline-5-carboxylate reductase (proC) CSB-YP746717SKV
CSB-EP746717SKV
CSB-BP746717SKV
CSB-MP746717SKV
CSB-EP746717SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus GTPase Der (der) CSB-YP746718SKV
CSB-EP746718SKV
CSB-BP746718SKV
CSB-MP746718SKV
CSB-EP746718SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Chorismate synthase (aroC) CSB-YP746719SKV
CSB-EP746719SKV
CSB-BP746719SKV
CSB-MP746719SKV
CSB-EP746719SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP746720SKV
CSB-EP746720SKV
CSB-BP746720SKV
CSB-MP746720SKV
CSB-EP746720SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0398 protein SAR1458 (SAR1458) CSB-YP746721SKV
CSB-EP746721SKV
CSB-BP746721SKV
CSB-MP746721SKV
CSB-EP746721SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Quinolone resistance protein norB (norB), partial CSB-YP746722SKV
CSB-EP746722SKV
CSB-BP746722SKV
CSB-MP746722SKV
CSB-EP746722SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Signal transduction histidine-protein kinase ArlS (arlS), partial CSB-YP746723SKV1
CSB-EP746723SKV1
CSB-BP746723SKV1
CSB-MP746723SKV1
CSB-EP746723SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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