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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Solute carrier family 25 member 33 (slc25a33), partial CSB-YP747384DIL1
CSB-EP747384DIL1
CSB-BP747384DIL1
CSB-MP747384DIL1
CSB-EP747384DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio CUGBP Elav-like family member 2 (celf2), partial CSB-YP747385DIL
CSB-EP747385DIL
CSB-BP747385DIL
CSB-MP747385DIL
CSB-EP747385DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable lipid phosphate phosphatase PPAPDC3 (ppapdc3), partial CSB-YP747386DIL1
CSB-EP747386DIL1
CSB-BP747386DIL1
CSB-MP747386DIL1
CSB-EP747386DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pleckstrin homology domain-containing family J member 1 (plekhj1) CSB-YP747387DIL
CSB-EP747387DIL
CSB-BP747387DIL
CSB-MP747387DIL
CSB-EP747387DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mis18-binding protein 1 (MIS18BP1), partial CSB-YP747388HU
CSB-EP747388HU
CSB-BP747388HU
CSB-MP747388HU
CSB-EP747388HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 39S ribosomal protein L54, mitochondrial (MRPL54) CSB-YP747389HU
CSB-EP747389HU
CSB-BP747389HU
CSB-MP747389HU
CSB-EP747389HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Structure-specific endonuclease subunit SLX4 (Slx4), partial CSB-YP747390MO
CSB-EP747390MO
CSB-BP747390MO
CSB-MP747390MO
CSB-EP747390MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform (Ppp2r2a) CSB-YP747391MO
CSB-EP747391MO
CSB-BP747391MO
CSB-MP747391MO
CSB-EP747391MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Armadillo repeat-containing X-linked protein 5 (ARMCX5) CSB-YP747392HU
CSB-EP747392HU
CSB-BP747392HU
CSB-MP747392HU
CSB-EP747392HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Myb/SANT-like DNA-binding domain-containing protein 2 (MSANTD2) CSB-YP747393HU
CSB-EP747393HU
CSB-BP747393HU
CSB-MP747393HU
CSB-EP747393HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic translation initiation factor 3 subunit C (eif3c), partial CSB-YP747394XBF
CSB-EP747394XBF
CSB-BP747394XBF
CSB-MP747394XBF
CSB-EP747394XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription initiation factor TFIID subunit 2 (TAF2), partial CSB-YP747395HU
CSB-EP747395HU
CSB-BP747395HU
CSB-MP747395HU
CSB-EP747395HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytosolic Fe-S cluster assembly factor nubp1 (nubp1) CSB-YP747396DIL
CSB-EP747396DIL
CSB-BP747396DIL
CSB-MP747396DIL
CSB-EP747396DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human X-ray radiation resistance-associated protein 1 (XRRA1) CSB-YP747397HU
CSB-EP747397HU
CSB-BP747397HU
CSB-MP747397HU
CSB-EP747397HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Histone-lysine N-methyltransferase NSD3 (Whsc1l1), partial CSB-YP747398MO
CSB-EP747398MO
CSB-BP747398MO
CSB-MP747398MO
CSB-EP747398MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Outer row dynein assembly protein 16 homolog (wdr69) CSB-YP747399XBF
CSB-EP747399XBF
CSB-BP747399XBF
CSB-MP747399XBF
CSB-EP747399XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Histone-binding protein RBBP7 (rbbp7) CSB-YP747400XBF
CSB-EP747400XBF
CSB-BP747400XBF
CSB-MP747400XBF
CSB-EP747400XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (sdhd), partial CSB-YP747401XBF1
CSB-EP747401XBF1
CSB-BP747401XBF1
CSB-MP747401XBF1
CSB-EP747401XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Estradiol 17-beta-dehydrogenase 12-A (hsd17b12a), partial CSB-YP747402DIL1
CSB-EP747402DIL1
CSB-BP747402DIL1
CSB-MP747402DIL1
CSB-EP747402DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SCY1-like protein 2 (SCYL2), partial CSB-YP747403HU
CSB-EP747403HU
CSB-BP747403HU
CSB-MP747403HU
CSB-EP747403HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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