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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis 30S ribosomal protein S19P (rps19p) CSB-YP747104MSB
CSB-EP747104MSB
CSB-BP747104MSB
CSB-MP747104MSB
CSB-EP747104MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Orotate phosphoribosyltransferase (pyrE) CSB-YP747105MSB
CSB-EP747105MSB
CSB-BP747105MSB
CSB-MP747105MSB
CSB-EP747105MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Prefoldin subunit alpha (pfdA) CSB-YP747106MSB
CSB-EP747106MSB
CSB-BP747106MSB
CSB-MP747106MSB
CSB-EP747106MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L30P (rpl30p) CSB-YP747107MSB
CSB-EP747107MSB
CSB-BP747107MSB
CSB-MP747107MSB
CSB-EP747107MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S17P (rps17p) CSB-YP747108MSB
CSB-EP747108MSB
CSB-BP747108MSB
CSB-MP747108MSB
CSB-EP747108MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Ribonuclease HII (rnhB) CSB-YP747109MSB
CSB-EP747109MSB
CSB-BP747109MSB
CSB-MP747109MSB
CSB-EP747109MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 50S ribosomal protein L13P (rpl13p) CSB-YP747110MSB
CSB-EP747110MSB
CSB-BP747110MSB
CSB-MP747110MSB
CSB-EP747110MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0200 protein MMP1282 (MMP1282) CSB-YP747111MSB
CSB-EP747111MSB
CSB-BP747111MSB
CSB-MP747111MSB
CSB-EP747111MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP747112MSB
CSB-EP747112MSB
CSB-BP747112MSB
CSB-MP747112MSB
CSB-EP747112MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Phosphate import ATP-binding protein PstB (pstB) CSB-YP747113MSB
CSB-EP747113MSB
CSB-BP747113MSB
CSB-MP747113MSB
CSB-EP747113MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Arginine--tRNA ligase (argS), partial CSB-YP747114MSB
CSB-EP747114MSB
CSB-BP747114MSB
CSB-MP747114MSB
CSB-EP747114MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis FAD synthase (ribL) CSB-YP747115MSB
CSB-EP747115MSB
CSB-BP747115MSB
CSB-MP747115MSB
CSB-EP747115MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0254 protein MMP0935 (MMP0935) CSB-YP747116MSB
CSB-EP747116MSB
CSB-BP747116MSB
CSB-MP747116MSB
CSB-EP747116MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP747117MSB
CSB-EP747117MSB
CSB-BP747117MSB
CSB-MP747117MSB
CSB-EP747117MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UPF0264 protein MMP0708 (MMP0708) CSB-YP747118MSB
CSB-EP747118MSB
CSB-BP747118MSB
CSB-MP747118MSB
CSB-EP747118MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S28e (rps28e) CSB-YP747119MSB
CSB-EP747119MSB
CSB-BP747119MSB
CSB-MP747119MSB
CSB-EP747119MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP747120MSB
CSB-EP747120MSB
CSB-BP747120MSB
CSB-MP747120MSB
CSB-EP747120MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein (MMP0415), partial CSB-YP747121MSB
CSB-EP747121MSB
CSB-BP747121MSB
CSB-MP747121MSB
CSB-EP747121MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP747122MSB
CSB-EP747122MSB
CSB-BP747122MSB
CSB-MP747122MSB
CSB-EP747122MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Nascent polypeptide-associated complex protein (nac) CSB-YP747123MSB
CSB-EP747123MSB
CSB-BP747123MSB
CSB-MP747123MSB
CSB-EP747123MSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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