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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP747304CQF
CSB-EP747304CQF
CSB-BP747304CQF
CSB-MP747304CQF
CSB-EP747304CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP747305CQF
CSB-EP747305CQF
CSB-BP747305CQF
CSB-MP747305CQF
CSB-EP747305CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP747306CQF
CSB-EP747306CQF
CSB-BP747306CQF
CSB-MP747306CQF
CSB-EP747306CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP747307CQF
CSB-EP747307CQF
CSB-BP747307CQF
CSB-MP747307CQF
CSB-EP747307CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamyl-tRNA reductase (hemA) CSB-YP747308CQF
CSB-EP747308CQF
CSB-BP747308CQF
CSB-MP747308CQF
CSB-EP747308CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant D-inositol 3-phosphate glycosyltransferase (mshA) CSB-YP747309CQF
CSB-EP747309CQF
CSB-BP747309CQF
CSB-MP747309CQF
CSB-EP747309CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chromosomal replication initiator protein DnaA (dnaA), partial CSB-YP747310CQF
CSB-EP747310CQF
CSB-BP747310CQF
CSB-MP747310CQF
CSB-EP747310CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AMSH-like ubiquitin thioesterase 2 (AMSH2) CSB-YP747311DOA
CSB-EP747311DOA
CSB-BP747311DOA
CSB-MP747311DOA
CSB-EP747311DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At5g39560 (At5g39560) CSB-YP747312DOA
CSB-EP747312DOA
CSB-BP747312DOA
CSB-MP747312DOA
CSB-EP747312DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g41720 (At5g41720) CSB-YP747313DOA
CSB-EP747313DOA
CSB-BP747313DOA
CSB-MP747313DOA
CSB-EP747313DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peptidyl-tRNA hydrolase, mitochondrial (At5g19830) CSB-YP747314DOA
CSB-EP747314DOA
CSB-BP747314DOA
CSB-MP747314DOA
CSB-EP747314DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-galactose/UDP-glucose transporter 5 (UTR5), partial CSB-YP747315DOA
CSB-EP747315DOA
CSB-BP747315DOA
CSB-MP747315DOA
CSB-EP747315DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase U12 (GSTU12) CSB-YP747316DOA
CSB-EP747316DOA
CSB-BP747316DOA
CSB-MP747316DOA
CSB-EP747316DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Alpha N-terminal protein methyltransferase 1 (CG1675) CSB-YP747317DLU
CSB-EP747317DLU
CSB-BP747317DLU
CSB-MP747317DLU
CSB-EP747317DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Root meristem growth factor 7 (RGF7) CSB-YP747318DOA
CSB-EP747318DOA
CSB-BP747318DOA
CSB-MP747318DOA
CSB-EP747318DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana WD repeat-containing protein DWA2 (DWA2) CSB-YP747319DOA
CSB-EP747319DOA
CSB-BP747319DOA
CSB-MP747319DOA
CSB-EP747319DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana AT-rich interactive domain-containing protein 4 (ARID4), partial CSB-YP747320DOA
CSB-EP747320DOA
CSB-BP747320DOA
CSB-MP747320DOA
CSB-EP747320DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NAC domain-containing protein 8 (NAC008) CSB-YP747321DOA
CSB-EP747321DOA
CSB-BP747321DOA
CSB-MP747321DOA
CSB-EP747321DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcriptional adapter 2-beta (tada2b) CSB-YP747322XBE
CSB-EP747322XBE
CSB-BP747322XBE
CSB-MP747322XBE
CSB-EP747322XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ankyrin repeat domain-containing protein 13C-A (ankrd13c-a), partial CSB-YP747323XBE
CSB-EP747323XBE
CSB-BP747323XBE
CSB-MP747323XBE
CSB-EP747323XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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