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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Claudin-1 (CLDN1), partial CSB-YP747024BO1
CSB-EP747024BO1
CSB-BP747024BO1
CSB-MP747024BO1
CSB-EP747024BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-glutamine:2-deoxy-scyllo-inosose aminotransferase (kanB) CSB-YP747025SGAD
CSB-EP747025SGAD
CSB-BP747025SGAD
CSB-MP747025SGAD
CSB-EP747025SGAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Circadian clock protein kaiB (kaiB) CSB-YP747026AVV
CSB-EP747026AVV
CSB-BP747026AVV
CSB-MP747026AVV
CSB-EP747026AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM72D (FAM72D) CSB-YP747027HU
CSB-EP747027HU
CSB-BP747027HU
CSB-MP747027HU
CSB-EP747027HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe A1 cistron-splicing factor aar2 (aar2) CSB-YP747028SXV
CSB-EP747028SXV
CSB-BP747028SXV
CSB-MP747028SXV
CSB-EP747028SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP747029OED
CSB-EP747029OED
CSB-BP747029OED
CSB-MP747029OED
CSB-EP747029OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararaca Bradykinin-potentiating and C-type natriuretic peptides CSB-YP747030BUW
CSB-EP747030BUW
CSB-BP747030BUW
CSB-MP747030BUW
CSB-EP747030BUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Serine/threonine transporter sstT (sstT), partial CSB-YP747031PIG
CSB-EP747031PIG
CSB-BP747031PIG
CSB-MP747031PIG
CSB-EP747031PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Regulatory protein recX (recX) CSB-YP747032PIG
CSB-EP747032PIG
CSB-BP747032PIG
CSB-MP747032PIG
CSB-EP747032PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Putative pterin-4-alpha-carbinolamine dehydratase (PBPRB1163) CSB-YP747033PIG
CSB-EP747033PIG
CSB-BP747033PIG
CSB-MP747033PIG
CSB-EP747033PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0060 membrane protein PBPRB0495 (PBPRB0495), partial CSB-YP747034PIG1
CSB-EP747034PIG1
CSB-BP747034PIG1
CSB-MP747034PIG1
CSB-EP747034PIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 5-dehydro-2-deoxygluconokinase (iolC) CSB-YP747035PIG
CSB-EP747035PIG
CSB-BP747035PIG
CSB-MP747035PIG
CSB-EP747035PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase subunit beta 2 (atpD2) CSB-YP747036PIG
CSB-EP747036PIG
CSB-BP747036PIG
CSB-MP747036PIG
CSB-EP747036PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum ATP synthase subunit b (atpF), partial CSB-YP747037PIG
CSB-EP747037PIG
CSB-BP747037PIG
CSB-MP747037PIG
CSB-EP747037PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP747038PIG
CSB-EP747038PIG
CSB-BP747038PIG
CSB-MP747038PIG
CSB-EP747038PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA threonylcarbamoyladenosine biosynthesis protein RimN (rimN) CSB-YP747039PIG
CSB-EP747039PIG
CSB-BP747039PIG
CSB-MP747039PIG
CSB-EP747039PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD), partial CSB-YP747040PIG
CSB-EP747040PIG
CSB-BP747040PIG
CSB-MP747040PIG
CSB-EP747040PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 50S ribosomal protein L10 (rplJ) CSB-YP747041PIG
CSB-EP747041PIG
CSB-BP747041PIG
CSB-MP747041PIG
CSB-EP747041PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Elongation factor P (efp) CSB-YP747042PIG
CSB-EP747042PIG
CSB-BP747042PIG
CSB-MP747042PIG
CSB-EP747042PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Oligoribonuclease (orn) CSB-YP747043PIG
CSB-EP747043PIG
CSB-BP747043PIG
CSB-MP747043PIG
CSB-EP747043PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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