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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Photobacterium profundum Probable allantoicase (alc) CSB-YP747064PIG
CSB-EP747064PIG
CSB-BP747064PIG
CSB-MP747064PIG
CSB-EP747064PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP747065PIG
CSB-EP747065PIG
CSB-BP747065PIG
CSB-MP747065PIG
CSB-EP747065PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Urocanate hydratase (hutU), partial CSB-YP747066PIG
CSB-EP747066PIG
CSB-BP747066PIG
CSB-MP747066PIG
CSB-EP747066PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Putative transport protein PBPRA2144 (PBPRA2144), partial CSB-YP747067PIG
CSB-EP747067PIG
CSB-BP747067PIG
CSB-MP747067PIG
CSB-EP747067PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0265 protein PBPRA1961 (PBPRA1961) CSB-YP747068PIG
CSB-EP747068PIG
CSB-BP747068PIG
CSB-MP747068PIG
CSB-EP747068PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Macrodomain Ter protein (matP) CSB-YP747069PIG
CSB-EP747069PIG
CSB-BP747069PIG
CSB-MP747069PIG
CSB-EP747069PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP747070PIG
CSB-EP747070PIG
CSB-BP747070PIG
CSB-MP747070PIG
CSB-EP747070PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phosphate import ATP-binding protein PstB 2 (pstB2) CSB-YP747071PIG
CSB-EP747071PIG
CSB-BP747071PIG
CSB-MP747071PIG
CSB-EP747071PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Methionine--tRNA ligase (metG), partial CSB-YP747072PIG
CSB-EP747072PIG
CSB-BP747072PIG
CSB-MP747072PIG
CSB-EP747072PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UvrABC system protein B (uvrB), partial CSB-YP747073PIG
CSB-EP747073PIG
CSB-BP747073PIG
CSB-MP747073PIG
CSB-EP747073PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum tRNA (mo5U34)-methyltransferase (cmoB) CSB-YP747074PIG
CSB-EP747074PIG
CSB-BP747074PIG
CSB-MP747074PIG
CSB-EP747074PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 7-cyano-7-deazaguanine synthase (queC) CSB-YP747075PIG
CSB-EP747075PIG
CSB-BP747075PIG
CSB-MP747075PIG
CSB-EP747075PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Periplasmic nitrate reductase 1 (napA1), partial CSB-YP747076PIG
CSB-EP747076PIG
CSB-BP747076PIG
CSB-MP747076PIG
CSB-EP747076PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0255 protein PBPRA0837 (PBPRA0837) CSB-YP747077PIG
CSB-EP747077PIG
CSB-BP747077PIG
CSB-MP747077PIG
CSB-EP747077PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP747078PIG
CSB-EP747078PIG
CSB-BP747078PIG
CSB-MP747078PIG
CSB-EP747078PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP747079PIG
CSB-EP747079PIG
CSB-BP747079PIG
CSB-MP747079PIG
CSB-EP747079PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP747080PIG
CSB-EP747080PIG
CSB-BP747080PIG
CSB-MP747080PIG
CSB-EP747080PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Cysteine desulfurase (iscS) CSB-YP747081PIG
CSB-EP747081PIG
CSB-BP747081PIG
CSB-MP747081PIG
CSB-EP747081PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP747082PIG
CSB-EP747082PIG
CSB-BP747082PIG
CSB-MP747082PIG
CSB-EP747082PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum Deoxyribose-phosphate aldolase (deoC) CSB-YP747083PIG
CSB-EP747083PIG
CSB-BP747083PIG
CSB-MP747083PIG
CSB-EP747083PIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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