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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Heterogeneous nuclear ribonucleoprotein D-like-B (hnrpdl-b) CSB-YP747344XBE
CSB-EP747344XBE
CSB-BP747344XBE
CSB-MP747344XBE
CSB-EP747344XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Serine/threonine-protein kinase TAO1-A (taok1-a), partial CSB-YP747345XBE
CSB-EP747345XBE
CSB-BP747345XBE
CSB-MP747345XBE
CSB-EP747345XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Chromosome transmission fidelity protein 18 homolog (chtf18), partial CSB-YP747346XBE
CSB-EP747346XBE
CSB-BP747346XBE
CSB-MP747346XBE
CSB-EP747346XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Methyltransferase-like protein 14 (mettl14) CSB-YP747347XBE
CSB-EP747347XBE
CSB-BP747347XBE
CSB-MP747347XBE
CSB-EP747347XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Ubiquitin thioesterase zranb1-B (zranb1-b), partial CSB-YP747348XBE
CSB-EP747348XBE
CSB-BP747348XBE
CSB-MP747348XBE
CSB-EP747348XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Roquin (rc3h1), partial CSB-YP747349XBE
CSB-EP747349XBE
CSB-BP747349XBE
CSB-MP747349XBE
CSB-EP747349XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PWWP domain-containing protein 2B (PWWP2B) CSB-YP747350HU
CSB-EP747350HU
CSB-BP747350HU
CSB-MP747350HU
CSB-EP747350HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RAD50-interacting protein 1 (RINT1) CSB-YP747351HU
CSB-EP747351HU
CSB-BP747351HU
CSB-MP747351HU
CSB-EP747351HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tectonic-3 (TCTN3), partial CSB-YP747352HU
CSB-EP747352HU
CSB-BP747352HU
CSB-MP747352HU
CSB-EP747352HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein (wfikkn), partial CSB-YP747353DIL
CSB-EP747353DIL
CSB-BP747353DIL
CSB-MP747353DIL
CSB-EP747353DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Non-homologous end-joining factor 1 (nhej1) CSB-YP747354DIL
CSB-EP747354DIL
CSB-BP747354DIL
CSB-MP747354DIL
CSB-EP747354DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane protein 41A-B (tmem41ab), partial CSB-YP747355DIL1
CSB-EP747355DIL1
CSB-BP747355DIL1
CSB-MP747355DIL1
CSB-EP747355DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse WD repeat-containing protein 44 (Wdr44), partial CSB-YP747356MO
CSB-EP747356MO
CSB-BP747356MO
CSB-MP747356MO
CSB-EP747356MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Bromodomain-containing protein 9 (brd9), partial CSB-YP747357XBF
CSB-EP747357XBF
CSB-BP747357XBF
CSB-MP747357XBF
CSB-EP747357XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis ATPase family AAA domain-containing protein 3 (atad3), partial CSB-YP747358XBF1
CSB-EP747358XBF1
CSB-BP747358XBF1
CSB-MP747358XBF1
CSB-EP747358XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Forkhead box protein C2 (foxc2) CSB-YP747359XBF
CSB-EP747359XBF
CSB-BP747359XBF
CSB-MP747359XBF
CSB-EP747359XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial (HIBCH) CSB-YP747360HU
CSB-EP747360HU
CSB-BP747360HU
CSB-MP747360HU
CSB-EP747360HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cyclic AMP-dependent transcription factor ATF-4 (atf4) CSB-YP747361DIL
CSB-EP747361DIL
CSB-BP747361DIL
CSB-MP747361DIL
CSB-EP747361DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cleavage and polyadenylation specificity factor subunit 6 (cpsf6), partial CSB-YP747362DIL
CSB-EP747362DIL
CSB-BP747362DIL
CSB-MP747362DIL
CSB-EP747362DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Keratin, type II cytoskeletal 8 (krt8), partial CSB-YP747363DIL
CSB-EP747363DIL
CSB-BP747363DIL
CSB-MP747363DIL
CSB-EP747363DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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