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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF61 (ORF61), partial CSB-YP747544OBAD
CSB-EP747544OBAD
CSB-BP747544OBAD
CSB-MP747544OBAD
CSB-EP747544OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF55 (ORF55) CSB-YP747545OBAD
CSB-EP747545OBAD
CSB-BP747545OBAD
CSB-MP747545OBAD
CSB-EP747545OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF48 (ORF48) CSB-YP747546OBAD
CSB-EP747546OBAD
CSB-BP747546OBAD
CSB-MP747546OBAD
CSB-EP747546OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF46 (ORF46) CSB-YP747547OBAD
CSB-EP747547OBAD
CSB-BP747547OBAD
CSB-MP747547OBAD
CSB-EP747547OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF41 (ORF41), partial CSB-YP747548OBAD
CSB-EP747548OBAD
CSB-BP747548OBAD
CSB-MP747548OBAD
CSB-EP747548OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF35 (ORF35) CSB-YP747549OBAD
CSB-EP747549OBAD
CSB-BP747549OBAD
CSB-MP747549OBAD
CSB-EP747549OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF31 (ORF30) CSB-YP747550OBAD
CSB-EP747550OBAD
CSB-BP747550OBAD
CSB-MP747550OBAD
CSB-EP747550OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Ribonucleoside-diphosphate reductase small chain (ORF20), partial CSB-YP747551OBAD
CSB-EP747551OBAD
CSB-BP747551OBAD
CSB-MP747551OBAD
CSB-EP747551OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Uncharacterized protein ORF15 (ORF15) CSB-YP747552OBAD
CSB-EP747552OBAD
CSB-BP747552OBAD
CSB-MP747552OBAD
CSB-EP747552OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ostreid herpesvirus 1 Putative primase (ORF7), partial CSB-YP747553OBAD
CSB-EP747553OBAD
CSB-BP747553OBAD
CSB-MP747553OBAD
CSB-EP747553OBAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphate transporter PHO1 homolog 8 (PHO1-H8), partial CSB-YP747554DOA1
CSB-EP747554DOA1
CSB-BP747554DOA1
CSB-MP747554DOA1
CSB-EP747554DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphate transporter PHO1 homolog 5 (PHO1-H5), partial CSB-YP747555DOA
CSB-EP747555DOA
CSB-BP747555DOA
CSB-MP747555DOA
CSB-EP747555DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenorhabdus nematophila Thymidylate synthase (thyA) CSB-YP747556XBH
CSB-EP747556XBH
CSB-BP747556XBH
CSB-MP747556XBH
CSB-EP747556XBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human WD repeat-containing protein 74 (WDR74) CSB-YP747557HU
CSB-EP747557HU
CSB-BP747557HU
CSB-MP747557HU
CSB-EP747557HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Presenilin-1 (PSEN1), partial CSB-YP747558DO
CSB-EP747558DO
CSB-BP747558DO
CSB-MP747558DO
CSB-EP747558DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon albiventer Cytochrome b (MT-CYB), partial CSB-YP747559AFAM
CSB-EP747559AFAM
CSB-BP747559AFAM
CSB-MP747559AFAM
CSB-EP747559AFAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Coiled-coil domain-containing protein 154 (Ccdc154) CSB-YP747560MO
CSB-EP747560MO
CSB-BP747560MO
CSB-MP747560MO
CSB-EP747560MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Short-chain dehydrogenase TIC 32, chloroplastic (TIC32) CSB-YP747561EWE
CSB-EP747561EWE
CSB-BP747561EWE
CSB-MP747561EWE
CSB-EP747561EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Fructose-1,6-bisphosphatase (fbp) CSB-YP747562DKK
CSB-EP747562DKK
CSB-BP747562DKK
CSB-MP747562DKK
CSB-EP747562DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbitpox virus DNA-directed RNA polymerase 35 kDa subunit (RPO35) CSB-YP747563RAD
CSB-EP747563RAD
CSB-BP747563RAD
CSB-MP747563RAD
CSB-EP747563RAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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