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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Cell division suppressor protein YneA (yneA) CSB-YP747944LAAD
CSB-EP747944LAAD
CSB-BP747944LAAD
CSB-MP747944LAAD
CSB-EP747944LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Tyrosine recombinase XerC (xerC) CSB-YP747945LAAD
CSB-EP747945LAAD
CSB-BP747945LAAD
CSB-MP747945LAAD
CSB-EP747945LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamate 5-kinase (proB) CSB-YP747946LAAD
CSB-EP747946LAAD
CSB-BP747946LAAD
CSB-MP747946LAAD
CSB-EP747946LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamate racemase (murI) CSB-YP747947LAAD
CSB-EP747947LAAD
CSB-BP747947LAAD
CSB-MP747947LAAD
CSB-EP747947LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP747948LAAD
CSB-EP747948LAAD
CSB-BP747948LAAD
CSB-MP747948LAAD
CSB-EP747948LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Cobyrinic acid A,C-diamide synthase (cobB) CSB-YP747949LAAD
CSB-EP747949LAAD
CSB-BP747949LAAD
CSB-MP747949LAAD
CSB-EP747949LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0223 protein LMOf2365_1079 (LMOf2365_1079) CSB-YP747950LAAD
CSB-EP747950LAAD
CSB-BP747950LAAD
CSB-MP747950LAAD
CSB-EP747950LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP747951LAAD
CSB-EP747951LAAD
CSB-BP747951LAAD
CSB-MP747951LAAD
CSB-EP747951LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0413 protein LMOf2365_0984 (LMOf2365_0984) CSB-YP747952LAAD
CSB-EP747952LAAD
CSB-BP747952LAAD
CSB-MP747952LAAD
CSB-EP747952LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP747953LAAD
CSB-EP747953LAAD
CSB-BP747953LAAD
CSB-MP747953LAAD
CSB-EP747953LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP747954LAAD
CSB-EP747954LAAD
CSB-BP747954LAAD
CSB-MP747954LAAD
CSB-EP747954LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 3-dehydroquinate dehydratase (aroD) CSB-YP747955LAAD
CSB-EP747955LAAD
CSB-BP747955LAAD
CSB-MP747955LAAD
CSB-EP747955LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Probable transcriptional regulatory protein LMOf2365_0385 (LMOf2365_0385) CSB-YP747956LAAD
CSB-EP747956LAAD
CSB-BP747956LAAD
CSB-MP747956LAAD
CSB-EP747956LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Thiamine-phosphate synthase (thiE) CSB-YP747957LAAD
CSB-EP747957LAAD
CSB-BP747957LAAD
CSB-MP747957LAAD
CSB-EP747957LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L1 (rplA) CSB-YP747958LAAD
CSB-EP747958LAAD
CSB-BP747958LAAD
CSB-MP747958LAAD
CSB-EP747958LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Lysine--tRNA ligase (lysS) CSB-YP747959LAAD
CSB-EP747959LAAD
CSB-BP747959LAAD
CSB-MP747959LAAD
CSB-EP747959LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Listeriolysin O (hly), partial CSB-YP747960LAAD
CSB-EP747960LAAD
CSB-BP747960LAAD
CSB-MP747960LAAD
CSB-EP747960LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Methionine--tRNA ligase (metG), partial CSB-YP747961LAAD
CSB-EP747961LAAD
CSB-BP747961LAAD
CSB-MP747961LAAD
CSB-EP747961LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative AgrB-like protein (LMOf2365_0057), partial CSB-YP747962LAAD1
CSB-EP747962LAAD1
CSB-BP747962LAAD1
CSB-MP747962LAAD1
CSB-EP747962LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Chromosomal replication initiator protein DnaA (dnaA) CSB-YP747963LAAD
CSB-EP747963LAAD
CSB-BP747963LAAD
CSB-MP747963LAAD
CSB-EP747963LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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