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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP747924LAAD
CSB-EP747924LAAD
CSB-BP747924LAAD
CSB-MP747924LAAD
CSB-EP747924LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 50S ribosomal protein L35 (rpmI) CSB-YP747925LAAD
CSB-EP747925LAAD
CSB-BP747925LAAD
CSB-MP747925LAAD
CSB-EP747925LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP747926LAAD
CSB-EP747926LAAD
CSB-BP747926LAAD
CSB-MP747926LAAD
CSB-EP747926LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Regulatory protein recX (recX) CSB-YP747927LAAD
CSB-EP747927LAAD
CSB-BP747927LAAD
CSB-MP747927LAAD
CSB-EP747927LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 30S ribosomal protein S2 (rpsB) CSB-YP747928LAAD
CSB-EP747928LAAD
CSB-BP747928LAAD
CSB-MP747928LAAD
CSB-EP747928LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Tryptophan synthase alpha chain (trpA) CSB-YP747929LAAD
CSB-EP747929LAAD
CSB-BP747929LAAD
CSB-MP747929LAAD
CSB-EP747929LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Tyrosine--tRNA ligase (tyrS) CSB-YP747930LAAD
CSB-EP747930LAAD
CSB-BP747930LAAD
CSB-MP747930LAAD
CSB-EP747930LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP747931LAAD
CSB-EP747931LAAD
CSB-BP747931LAAD
CSB-MP747931LAAD
CSB-EP747931LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Acetate kinase 2 (ackA2) CSB-YP747932LAAD
CSB-EP747932LAAD
CSB-BP747932LAAD
CSB-MP747932LAAD
CSB-EP747932LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Threonine--tRNA ligase (thrS), partial CSB-YP747933LAAD
CSB-EP747933LAAD
CSB-BP747933LAAD
CSB-MP747933LAAD
CSB-EP747933LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Valine--tRNA ligase (valS), partial CSB-YP747934LAAD
CSB-EP747934LAAD
CSB-BP747934LAAD
CSB-MP747934LAAD
CSB-EP747934LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0758 protein LMOf2365_1569 (LMOf2365_1569) CSB-YP747935LAAD
CSB-EP747935LAAD
CSB-BP747935LAAD
CSB-MP747935LAAD
CSB-EP747935LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Transcription elongation factor GreA (greA) CSB-YP747936LAAD
CSB-EP747936LAAD
CSB-BP747936LAAD
CSB-MP747936LAAD
CSB-EP747936LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b GTPase Era (era) CSB-YP747937LAAD
CSB-EP747937LAAD
CSB-BP747937LAAD
CSB-MP747937LAAD
CSB-EP747937LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Pyruvate formate-lyase-activating enzyme (pflA) CSB-YP747938LAAD
CSB-EP747938LAAD
CSB-BP747938LAAD
CSB-MP747938LAAD
CSB-EP747938LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative competence-damage inducible protein (cinA) CSB-YP747939LAAD
CSB-EP747939LAAD
CSB-BP747939LAAD
CSB-MP747939LAAD
CSB-EP747939LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Arginine repressor (argR) CSB-YP747940LAAD
CSB-EP747940LAAD
CSB-BP747940LAAD
CSB-MP747940LAAD
CSB-EP747940LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP747941LAAD
CSB-EP747941LAAD
CSB-BP747941LAAD
CSB-MP747941LAAD
CSB-EP747941LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Proline--tRNA ligase (proS), partial CSB-YP747942LAAD
CSB-EP747942LAAD
CSB-BP747942LAAD
CSB-MP747942LAAD
CSB-EP747942LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Uridylate kinase (pyrH) CSB-YP747943LAAD
CSB-EP747943LAAD
CSB-BP747943LAAD
CSB-MP747943LAAD
CSB-EP747943LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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