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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF146 (ORF146) CSB-YP747844AFAE
CSB-EP747844AFAE
CSB-BP747844AFAE
CSB-MP747844AFAE
CSB-EP747844AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF221 (ORF221), partial CSB-YP747845AFAE
CSB-EP747845AFAE
CSB-BP747845AFAE
CSB-MP747845AFAE
CSB-EP747845AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF426 (ORF426) CSB-YP747846AFAE
CSB-EP747846AFAE
CSB-BP747846AFAE
CSB-MP747846AFAE
CSB-EP747846AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF59b (ORF59b) CSB-YP747847AFAE
CSB-EP747847AFAE
CSB-BP747847AFAE
CSB-MP747847AFAE
CSB-EP747847AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidianus filamentous virus 1 Uncharacterized protein ORF190 (ORF190) CSB-YP747848AFAE
CSB-EP747848AFAE
CSB-BP747848AFAE
CSB-MP747848AFAE
CSB-EP747848AFAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Peptidoglycan-recognition protein SB2 (PGRP-SB2) CSB-YP747849DMJ
CSB-EP747849DMJ
CSB-BP747849DMJ
CSB-MP747849DMJ
CSB-EP747849DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inia geoffrensis NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP747850IKR1
CSB-EP747850IKR1
CSB-BP747850IKR1
CSB-MP747850IKR1
CSB-EP747850IKR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Balaena mysticetus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP747851BSA1
CSB-EP747851BSA1
CSB-BP747851BSA1
CSB-MP747851BSA1
CSB-EP747851BSA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis cosyra Alcohol dehydrogenase 1 (ADH1) CSB-YP747852CHAQ
CSB-EP747852CHAQ
CSB-BP747852CHAQ
CSB-MP747852CHAQ
CSB-EP747852CHAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus multicinctus Neurotoxin BM10-1-like CSB-YP747853BXN
CSB-EP747853BXN
CSB-BP747853BXN
CSB-MP747853BXN
CSB-EP747853BXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenolestes fuliginosus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP747854CAD1
CSB-EP747854CAD1
CSB-BP747854CAD1
CSB-MP747854CAD1
CSB-EP747854CAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP747855AIQ1
CSB-EP747855AIQ1
CSB-BP747855AIQ1
CSB-MP747855AIQ1
CSB-EP747855AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant NAD (P)H-quinone oxidoreductase subunit 4L, chloroplastic (ndhE), partial CSB-YP747856AIQ1
CSB-EP747856AIQ1
CSB-BP747856AIQ1
CSB-MP747856AIQ1
CSB-EP747856AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14, chloroplastic (rpl14) CSB-YP747857AIQ
CSB-EP747857AIQ
CSB-BP747857AIQ
CSB-MP747857AIQ
CSB-EP747857AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L33, chloroplastic (rpl33) CSB-YP747858AIQ
CSB-EP747858AIQ
CSB-BP747858AIQ
CSB-MP747858AIQ
CSB-EP747858AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit beta, chloroplastic (atpB) CSB-YP747859AIQ
CSB-EP747859AIQ
CSB-BP747859AIQ
CSB-MP747859AIQ
CSB-EP747859AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem I assembly protein ycf3 (ycf3) CSB-YP747860AIQ
CSB-EP747860AIQ
CSB-BP747860AIQ
CSB-MP747860AIQ
CSB-EP747860AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II CP43 chlorophyll apoprotein (psbC), partial CSB-YP747861AIQ1
CSB-EP747861AIQ1
CSB-BP747861AIQ1
CSB-MP747861AIQ1
CSB-EP747861AIQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photosystem II reaction center protein K (psbK) CSB-YP747862AIQ
CSB-EP747862AIQ
CSB-BP747862AIQ
CSB-MP747862AIQ
CSB-EP747862AIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Euphractus sexcinctus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP747863ECAR
CSB-EP747863ECAR
CSB-BP747863ECAR
CSB-MP747863ECAR
CSB-EP747863ECAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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